Complete human serum maintains viability and chondrogenic potential of human synovial stem cells: suitable conditions for transplantation.
Complete human serum maintains viability and chondrogenic potential of human synovial stem cells: suitable conditions for transplantation.
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DOI：
10.1186/s13287-017-0596-0
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发表时间：
2017-06-13
影响因子：
7.5
通讯作者：
Sekiya I
中科院分区：
文献类型：
--
作者：
Mizuno M;Katano H;Otabe K;Komori K;Kohno Y;Fujii S;Ozeki N;Horie M;Tsuji K;Koga H;Muneta T;Sekiya I
在我们的临床实践中，我们进行自体滑膜间充质干细胞（MSCs）的软骨和半月板再生医学移植。确保临床疗效的最重要问题之一涉及滑膜MSC从加工设施到诊所的运输。完整的人血清（100%人血清）是一种有吸引力的候选材料，其中悬浮滑膜MSC用于在运输过程中保存。本研究的目的是调查是否完整的人血清保持MSC的活力和软骨形成的潜力，并检查最佳的温度条件下保存的人滑膜间充质干细胞。在全膝关节置换术中，从14名患有骨关节炎的供体的膝关节中获取人类滑膜。将第2代滑膜MSC以200万个细胞/100 μL悬浮于林格氏溶液或完全人血清中，在4、13和37 °C下持续48小时。在保存前后分析这些细胞的活细胞率、细胞表面标记物表达、代谢活性、增殖和成脂、钙化和软骨形成分化潜力。保存后，在4 ° C和13 °C下，滑膜MSC在人血清中的活细胞率高于林格氏溶液。在4 ° C和13 °C下保存在人血清中的滑膜MSC也保持高比率的碘化丙啶-和膜联蛋白V-细胞。MSC表面标志物表达在4和13 °C保存的细胞中没有改变。在4 ° C和13 °C下保存在人血清中的细胞的代谢活性得以维持，而在其他条件下显著降低。当在4 ° C和13 °C下保存在人血清中时，再接种的MSC保留其增殖能力。在每种条件下保存的细胞中均可观察到脂肪生成和钙化潜力，而软骨形成潜力仅保留在4和13 °C下保存于人血清中的细胞中。当细胞在4 ° C和13 °C下悬浮于人血清中时，滑膜MSC的活力和软骨形成潜力得以维持。本文的在线版本（doi：10.1186/s13287-017-0596-0）包含补充材料，可供授权用户使用。
In our clinical practice, we perform transplantations of autologous synovial mesenchymal stem cells (MSCs) for cartilage and meniscus regenerative medicine. One of the most important issues to ensuring clinical efficacy involves the transport of synovial MSCs from the processing facility to the clinic. Complete human serum (100% human serum) is an attractive candidate material in which to suspend synovial MSCs for their preservation during transport. The purpose of this study was to investigate whether complete human serum maintained MSC viability and chondrogenic potential and to examine the optimal temperature conditions for the preservation of human synovial MSCs. Human synovium was harvested from the knees of 14 donors with osteoarthritis during total knee arthroplasty. Passage 2 synovial MSCs were suspended at 2 million cells/100 μL in Ringer’s solution or complete human serum at 4, 13, and 37 °C for 48 h. These cells were analyzed for live cell rates, cell surface marker expression, metabolic activity, proliferation, and adipogenic, calcification, and chondrogenic differentiation potentials before and after preservation. After preservation, synovial MSCs maintained higher live cell rates in human serum than in Ringer’s solution at 4 and 13 °C. Synovial MSCs preserved in human serum at 4 and 13 °C also maintained high ratios of propidium iodide– and annexin V– cells. MSC surface marker expression was not altered in cells preserved at 4 and 13 °C. The metabolic activities of cells preserved in human serum at 4 and 13 °C was maintained, while significantly reduced in other conditions. Replated MSCs retained their proliferation ability when preserved in human serum at 4 and 13 °C. Adipogenesis and calcification potential could be observed in cells preserved in each condition, whereas chondrogenic potential was retained only in cells preserved in human serum at 4 and 13 °C. The viability and chondrogenic potential of synovial MSCs were maintained when the cells were suspended in human serum at 4 and 13 °C. The online version of this article (doi:10.1186/s13287-017-0596-0) contains supplementary material, which is available to authorized users.