Nonsurgical Embryo Transfer Protocol for Use with the NSET™ Device.
Nonsurgical Embryo Transfer Protocol for Use with the NSET™ Device.
复制标题
适用于 NSET™ 设备的非手术胚胎移植协议。
DOI：
10.1007/978-1-4939-9837-1_9
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Stone,BarbaraJ
中科院分区：
文献类型：
--
作者：
Stone,BarbaraJ
转基因胚胎必须转移到合适的雌性受体，以便发育成幼崽。非手术胚胎移植是一种快速有效的方法，用于将囊胚期胚胎运送到受体女性的子宫角。非手术胚胎移植后幼仔的恢复效率与手术移植后的效率相似。然而，非手术转移消除了手术过程引起的疼痛和痛苦，并根据Russel和Burch的“3Rs”（人道实验技术的原则）提供了一种改进。梅图恩公司，伦敦，1959年），动物研究的伦理框架。该方法也可用于小鼠品系的再衍生。再衍生对于在运输后从传入小鼠品系中去除潜在病原体或在设施内获得干净的小鼠菌落非常重要。
Genetically modified embryos must be transferred to a suitable female recipient for development to pups. Nonsurgical embryo transfer is a fast and efficient method used to deliver blastocyst stage embryos to the uterine horn of recipient females. The efficiency of recovery of pups after nonsurgical embryo transfer is similar to the efficiency after surgical transfer. However, nonsurgical transfer eliminates the pain and distress caused by the surgical procedure and provides a refinement in accordance with Russel and Burch’s “3Rs” (The principles of humane experimental technique. Methuen & Co., London, 1959), an ethical framework for animal research. This method is also useful for rederivation of mouse strains. Rederivation is important for either removal of potential pathogens from an incoming mouse strain after shipping, or within a facility to obtain a clean mouse colony.