Transcriptional expression levels of chicken collectins are affected by avian influenza A virus inoculation
Transcriptional expression levels of chicken collectins are affected by avian influenza A virus inoculation
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DOI：
10.1016/j.vetmic.2009.09.026
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发表时间：
2010-03-24
影响因子：
3.3
通讯作者：
Vervelde, Lonneke
中科院分区：
文献类型：
--
作者：
Reemers, Sylvia S.;Veldhuizen, Edwin J. A.;Vervelde, Lonneke
已发现哺乳动物集合素在防御甲型流感病毒 H9N2 接种中发挥重要作用，但对于鸡集合素而言，这一点尚未得到澄清。本研究的目的是确定甲型禽流感病毒 (AIV) 接种对鸡呼吸道集合素基因表达的影响，以及这是否受年龄影响。为此，对 1 周龄和 4 周龄的鸡进行气管内接种 PBS 或 H9N2 AIV。在接种后 0、8、16 和 24 小时处死鸡，并收获气管和肺进行分析。采用实时定量 RT-PCR 测定病毒 RNA 表达以及鸡集合素 1 和 2（cCL-1 和 cCL-2）、鸡肺凝集素（cLL）和鸡表面活性蛋白 A（cSP-A）的 mRNA 表达。在肺部，接种 H9N2 后，1 周龄和 4 周龄的鸡只观察到 cCL-2、cLL 和 cSP-A mRNA 表达下降，尽管时间点不同。而在气管中，仅在 4 周龄的鸡中观察到变化，并且表达增加。 1周龄鸡肺中collectin的表达与病毒RNA的表达相关。这些结果表明，年龄和呼吸道位置都会影响接种 H9N2 后集合素 mRNA 表达的变化，并表明集合素在鸡呼吸道中宿主对 AIV 的反应中可能发挥作用。 (C) 2009 Elsevier B.V. 保留所有权利。
Mammalian collect-ins have been found to play an important role in the defense against influenza A virus H9N2 inoculation, but for chicken collectins this has not yet been clarified. The aim of this study was to determine the effect of avian influenza A virus (AIV) inoculation on collectin gene expression in the respiratory tract of chickens and whether this was affected by age For this purpose 1- and 4-week-old chickens were inoculated intratracheally with PBS or H9N2 AIV Chickens were killed at 0, 8, 16 and 24 h post-inoculation and trachea and lung were harvested for analysis. Viral RNA expression and mRNA expression of chicken collectins 1 and 2 (cCL-1 and cCL-2), chicken lung lectin (cLL) and chicken surfactant protein A (cSP-A) were determined using real-time quantitative RT-PCR. In lung, a decrease in mRNA expression of cCL-2, cLL and cSP-A after inoculation with H9N2 was seen in both 1- and 4-week-old birds, although at different time points. while in trachea changes were only seen in 4-week-old birds and expression was increased Moreover. collectin expression correlated with viral RNA expression in lung of 1-week-old birds. These results suggest that both age and location in the respiratory tract affect changes in collectin mRNA expression after inoculation with H9N2 and indicate a possible role for collectins in the host response to AIV in the respiratory tract of chickens. (C) 2009 Elsevier B.V. All rights reserved.