Conservation and expression of IQ-domain-containing calpacitin gene products (neuromodulin/GAP-43, neurogranin/RC3) in the adult and developing oscine song control system.
Conservation and expression of IQ-domain-containing calpacitin gene products (neuromodulin/GAP-43, neurogranin/RC3) in the adult and developing oscine song control system.
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DOI：
10.1002/dneu.20686
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发表时间：
2009-02-01
影响因子：
3
通讯作者：
Siepka SM
中科院分区：
文献类型：
--
作者：
Clayton DF;George JM;Mello CV;Siepka SM
鸣禽因其对调节神经可塑性的见解而受到赞赏。在这里，我们描述的比较分析和大脑表达的两个基因序列编码可能的调节突触可塑性鸣禽：神经调节素（GAP-43）和神经颗粒素（RC 3）。两者都是calpacitin家族的成员，并且共享一个独特的保守核心结构域，该结构域介导钙、钙调蛋白和蛋白激酶C信号通路之间的相互作用。比较序列分析与已知的系统发育关系是一致的，鸣禽与鸡的关系最密切，与哺乳动物和鱼类的关系越来越远。神经颗粒蛋白的C-末端在鸟类和哺乳动物中是不同的，并且该蛋白的抗体显示在成年斑胸草雀小脑浦肯野细胞中的高表达，这在其他物种中尚未观察到。这两种蛋白质的RNA通常在端脑中丰富，但在成年金丝雀和斑胸草雀的歌曲控制系统的某些核中显着减少：神经调节素RNA在RA和HVC中非常低（相对于周围的苍白球区域），而神经颗粒素RNA在X区域中显着低（相对于周围的纹状体）。在这两种情况下，这种选择性的下调在斑胸草雀的幼年歌曲学习期间，孵化后25-45天。这些结果表明，分子平行的成年鸟类的歌曲控制电路的鲁棒稳定性。
Songbirds are appreciated for the insights they provide into regulated neural plasticity. Here we describe the comparative analysis and brain expression of two gene sequences encoding probable regulators of synaptic plasticity in songbirds: Neuromodulin (GAP-43) and Neurogranin (RC3). Both are members of the calpacitin family and share a distinctive conserved core domain that mediates interactions between calcium, calmodulin and protein kinase C signaling pathways. Comparative sequence analysis is consistent with known phylogenetic relationships, with songbirds most closely related to chicken and progressively more distant from mammals and fish. The C-terminus of Neurogranin is different in birds and mammals, and antibodies to the protein reveal high expression in adult zebra finches in cerebellar Purkinje cells, which has not been observed in other species. RNAs for both proteins are generally abundant in the telencephalon yet markedly reduced in certain nuclei of the song control system in adult canaries and zebra finches: Neuromodulin RNA is very low in RA and HVC (relative to the surrounding pallial areas), whereas Neurogranin RNA is conspicuously low in Area X (relative to surrounding striatum). In both cases, this selective down-regulation develops in the zebra finch during the juvenile song learning period, 25–45 days after hatching. These results suggest molecular parallels to the robust stability of the adult avian song control circuit.