Revisiting the phosphatidylethanolamine-binding protein (PEBP) gene family reveals cryptic FLOWERING LOCUS T gene homologs in gymnosperms and sheds new light on functional evolution
Revisiting the phosphatidylethanolamine-binding protein (PEBP) gene family reveals cryptic FLOWERING LOCUS T gene homologs in gymnosperms and sheds new light on functional evolution
复制标题
重新审视磷脂酰乙醇胺结合蛋白 (PEBP) 基因家族揭示了裸子植物中神秘的 FLOWERING LOCUS T 基因同源物，并为功能进化提供了新的线索
DOI：
10.1111/nph.14066
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发表时间：
2016-11-01
期刊：
影响因子：
9.4
通讯作者：
Wang, Xiao-Quan
中科院分区：
文献类型：
--
作者：
Liu, Yan-Yan;Yang, Ke-Zhen;Wang, Xiao-Quan
被子植物和裸子植物是现存种子植物的两大类群。裸子植物缺乏开花位点T（开花位点T），这是被子植物开花途径的核心关键整合子。利用新发布的裸子植物基因组，通过系统发育重建，重新审视了植物磷脂酰乙醇胺结合蛋白（PEBP）基因家族的进化历史。研究了裸子植物FT-like基因和TFL1 (TERMINAL FLOWER 1)-like基因在3个裸子植物分类群中的表达模式以及在拟南芥中的异源表达。系统发育重建表明，在种子植物分化之前，一个古老的基因重复产生了FT和TFL1基因。表达模式表明，裸子植物tfl1样基因在生殖发育过程中发挥作用，而裸子植物共同祖先复制事件产生的ft样基因GymFT1和GymFT2在生长节律和性发育途径中都起作用。当在拟南芥中表达时，云杉ft样基因和tfl1样基因都抑制开花。我们的研究表明裸子植物确实有ft样基因和tfl1样基因。频繁的基因和基因组重复对植物PEBP基因家族的扩展起着重要的作用。裸子植物PEBP基因的表达模式为该基因家族的功能进化提供了新的视角。
Angiosperms and gymnosperms are two major groups of extant seed plants. It has been suggested that gymnosperms lack FLOWERING LOCUS T (FT), a key integrator at the core of flowering pathways in angiosperms. Taking advantage of newly released gymnosperm genomes, we revisited the evolutionary history of the plant phosphatidylethanolamine-binding protein (PEBP) gene family through phylogenetic reconstruction. Expression patterns in three gymnosperm taxa and heterologous expression in Arabidopsis were studied to investigate the functions of gymnosperm FT-like and TERMINAL FLOWER 1 (TFL1)-like genes. Phylogenetic reconstruction suggests that an ancient gene duplication predating the divergence of seed plants gave rise to the FT and TFL1 genes. Expression patterns indicate that gymnosperm TFL1-like genes play a role in the reproductive development process, while GymFT1 and GymFT2, the FT-like genes resulting from a duplication event in the common ancestor of gymnosperms, function in both growth rhythm and sexual development pathways. When expressed in Arabidopsis, both spruce FT-like and TFL1-like genes repressed flowering. Our study demonstrates that gymnosperms do have FT-like and TFL1-like genes. Frequent gene and genome duplications contributed significantly to the expansion of the plant PEBP gene family. The expression patterns of gymnosperm PEBP genes provide novel insight into the functional evolution of this gene family.