Direct DNA sequencing of PCR products
Direct DNA sequencing of PCR products
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PCR 产物的直接 DNA 测序
DOI：
10.1002/em.2850180413
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发表时间：
1991
影响因子：
2.8
通讯作者：
J. Fuscoe
中科院分区：
文献类型：
--
作者：
L. Zimmerman;J. Fuscoe
描述了阐明与聚合酶链式反应（PCR）扩增的DNA的直接测序相关的一些技术问题的实验。测序引物纯度，标记方法，和模板制备进行了探索。提供了单链和双链PCR产物常规测序的条件。
Experiments are described which elucidate some of the technical problems associated with the direct sequencing of polymerase chain reaction (PCR) amplified DNA. Sequencing primer purity, labeling methodology, and template preparation were explored. Conditions are presented for the routine sequencing of single‐ and double‐stranded PCR products.