Effect of high fat diet on body weight and mammary tumor latency in MMTV-TGF-α mice
Effect of high fat diet on body weight and mammary tumor latency in MMTV-TGF-α mice
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DOI：
10.1038/sj.ijo.0802664
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发表时间：
2004-08-01
影响因子：
4.9
通讯作者：
Maihle, NJ
中科院分区：
文献类型：
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作者：
Cleary, MP;Grande, JP;Maihle, NJ
目的：高脂肪饮食在乳腺癌/乳腺肿瘤（MT）发展中的作用存在争议。这可能部分归因于高脂肪饮食对体重的不同影响。在此，我们使用一种中等高脂肪饮食（32.5%的脂肪热量），预计这种饮食会使大多数小鼠肥胖，但也预计会有一些小鼠的体重保持在低脂饮食（11%的脂肪热量）喂养的小鼠的体重范围内。这为比较食用高脂肪饮食但体重不同的小鼠以及体重相似但食用高脂肪和低脂饮食的小鼠提供了机会。
实验方法：转基因MMTV - TGF -α小鼠是一种绝经后乳腺癌模型，从10周龄开始，一组食用低脂饮食，即常规饲料喂养（n = 25），另一组食用中等高脂肪饮食（n = 51）。以34周龄时的体重将高脂肪饮食小鼠分为易肥胖组>超重组>抗肥胖组（n = 17）（P超重>抗肥胖 = 常规饲料喂养小鼠。脂肪垫和脂肪垫与胴体的比值在易肥胖组最重，其次是超重组小鼠。然而，抗肥胖组小鼠的脂肪垫重量以及脂肪垫与胴体的比值是常规饲料喂养小鼠的三倍。所有组的乳腺肿瘤发病率在72% - 82%之间。易肥胖组小鼠的乳腺肿瘤潜伏期最短（P（此处似乎原文未完整）
OBJECTIVE: The role of high fat diets in breast cancer/mammary tumor (MT) development is controversial. This may be partially attributable to variable effects of high fat diets on body weight. Here, we used a moderately high fat diet (32.5% fat calories) expected to cause obesity in most mice, but predicted to result in some mice remaining in the weight range of mice fed the low fat diet (11% fat calories). This provided the opportunity to compare mice fed the high fat diet exhibiting different body weights and mice of similar weight consuming high vs low fat diets.EXPERIMENTAL METHODS: Transgenic MMTV-TGF-alpha mice, a model of postmenopausal breast cancer, consumed a low fat diet, that is, chow-fed ( n = 25) or a moderately high fat diet from 10 weeks of age ( n = 51). Body weight at 34 weeks of age was used to assign high fat diet mice to obesity-prone > overweight > obesity-resistant groups ( n = 17) ( P overweight > obesity-resistant = chow-fed mice. Fat pads and fat pad: carcass were heaviest in obesity-prone followed by overweight mice. However, obesity-resistant mice had fat pad weights and fat pad: carcass three-fold greater than chow-fed mice. All groups had MT incidences between 72 and 82%. Obesity-prone mice exhibited the shortest MT latency ( P