Study of genetic reassortment between two human rotaviruses.
Study of genetic reassortment between two human rotaviruses.
复制标题
两种人类轮状病毒之间的基因重排研究。
DOI：
10.1016/0042-6822(84)90381-7
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
J. Nicolas
中科院分区：
文献类型：
--
作者：
A. Garbarg‐Chenon;F. Bricout;J. Nicolas
八种不同的人轮状病毒株适应在细胞培养中复制。在体外，轮状病毒基因重排已在两种病毒株之间进行，一种具有长模式的节段基因组RNA，另一种具有短模式的基因组RNA电泳迁移率。尽管在亲本菌株中缺乏选择性标记，但这种遗传重配是可检测的。此外，一些重组病毒表现出混合RNA模式，含有额外的基因组RNAS类似于临床标本中发现的那些。这些混合RNA带型的病毒在细胞培养中连续传代后可分离出其补充片段。最后，已经表明，在长和短电泳型中的基因组片段的分类不仅应该基于它们的相对大小，而且还应该基于它们与遗传上等同的片段重新分配的能力。
Eight different human rotavirus strains were adapted to replicate in cell culture.In vitro, rotavirus genetic reassortment has been performed between two virus strains, one with a long pattern of segmented genomic RNAs and the other with a short pattern in the electrophoretic mobility of genomic RNAs. This genetic reassortment was detectable despite the lack of selective markers in the parental strains. Moreover, some of the reassorted virus exhibited mixed RNA patterns containing extra genomic RNAS resembling those found in clinical specimens. These viruses with mixed RNA band patterns could segregate their supplementary segments after successive passages in cell culture. Finally it has been shown that classification of genomic segments in long and short electropherotypes should not only be based on their relative size but also by their ability to reassort with the genetically equivalent segment.