[Chinmedomics: a new strategy for research of traditional Chinese medicine].
[Chinmedomics: a new strategy for research of traditional Chinese medicine].
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DOI：
10.4268/cjcmm20150401
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发表时间：
2015-02
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中科院分区：
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证候和方剂是中医的两个关键问题，是中医物质基础研究的前提。然而证候的模糊性和方剂的复杂性极大地限制了对证候的评价和处方的有效物质基础。因此，如何解决证候评价、确定处方疗效物质基础是当前国际关注的热点问题。针对这些问题，将中药血清药物化学与代谢组学技术相结合建立中药组学这一中药研究的独特方法，为解决中药有效性和安全性等国际关注问题做出了突出贡献。在证候生物学特征的基础上，建立中医证候动物模型的代谢谱、相关代谢指纹及代谢生物标志物，评价中药方剂的整体疗效及证方对应关系。使用（内源性）标记代谢物和（外源性）血清成分（PCMS）之间的相关性绘图来筛选活性成分，并且正在通过进一步的生物学实验进行验证。口服配方后体内成分与体内内源性标志物的相关性分析，可明确活性成分及协同特性。该方法成功应用于中药中潜在生物活性成分和代谢物的快速发现，并通过一系列中药组学研究，证明所建立的方法有助于发掘中药进一步研究的有效物质。中药组学作为一种新的研究方法，是最符合中医整体观念的方法，它不仅可以解释证候的本质，而且可以阐明中药方剂的科学内涵。
Syndrome and formulae (or prescription) are two key issues in traditional Chinese medicine (TCM) and the premise research for material basis of TCM. However, vagueness of syndromes and complexity of formulae greatly limited the evaluation to syndromes and effective substance basis of prescription. Therefore, how to solve the evaluation of syndromes, confirming the efficacy material basis in prescription are the current hot issues of international concern. To solve these problems, establishing chinmedomics by integrated serum pharmacochemistry of TCM with metabolomics technology, that is a unique method of TCM research, made outstanding contributions in solving international concerns such as the effectiveness and security aspects of TCM. On the basis of the biological characterization of syndrome, the metabolic profiling of animal models of TCM syndrome, and related metabolic fingerprints as well as metabolic biomarkers were established to evaluate the overall effects of TCM formulae and corresponding relationship of syndrome-formulae. The active constituents were screened using the plotting of correlation between (endogenous) marker metabolites and (exogenous) serum constituents (PCMS), and is ongoing verification by further biological experiments. Correlation analysis between the ingredients in the body after oral formulae and endogenous markers in vivo can be used to clarify the active ingredients and synergistic properties. This method was successfully applied for rapid discovery of potentially bioactive components and metabolites from TCM, and through a series of studies on the chinmedomics, it proved that the established method could help to explore the effective substance for further research of TCM. As a new research approach, Chinmedomics is the best method to fit the holistic concept of TCM, and it can not only interpret the essence of syndrome but also elucidate the scientific connotation of Chinese medical formulae.