Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography
Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography
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DOI：
10.1016/j.febslet.2007.09.036
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发表时间：
2007-10-16
期刊：
影响因子：
3.5
通讯作者：
Shen, Jian-Ren
中科院分区：
文献类型：
--
作者：
Kawakami, Keisuke;Iwai, Masako;Shen, Jian-Ren
PsbY是光合放氧系统11（PSII）的低分子量亚基之一。然而，它的位置在PSII目前的晶体结构中还没有被确定。我们构建了一个PsbY-缺失突变体的细长热聚球藻，结晶，并分析了晶体结构的突变PSII二聚体。结果表明，PsbY位于PSII的外周，靠近细胞色素b559的α-和β-亚基，这对应于T. vulcanus或螺旋X2的3.0埃结构。细长的我们的研究结果还表明，PsbY的C-末端环突出向间质侧，而不是管腔侧先前预测。(c)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem 11 (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII dimer. The results obtained showed that PsbY is located in the periphery of PSII close to the alpha- and beta-subunits of cytochrome b559, which corresponded to an unassigned helix in the 3.7 angstrom structure of T. vulcanus or helix X2 in the 3.0 angstrom structure of T. elongatus. Our results also indicated that the C-terminal loop of PsbY is protruded toward the stromal side, instead of the lumenal side predicted previously. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.