Chemical and Genetic Study of Ligularia duciformis and Related Species in Sichuan and Yunnan Provinces of China
Chemical and Genetic Study of Ligularia duciformis and Related Species in Sichuan and Yunnan Provinces of China
复制标题
DOI：
10.1002/cbdv.201100240
复制
发表时间：
2012-04-01
影响因子：
2.9
通讯作者：
Kuroda, Chiaki
中科院分区：
文献类型：
--
作者：
Nagano, Hajime;Hanai, Ryo;Kuroda, Chiaki
对采自中国四川省和云南省的 28 个橐吾、空卡林橐吾和莲叶橐吾样品的根提取物的化学成分和进化中性 DNA 碱基序列进行了研究。这些样本可分为四种化学型 (14)。分别从两个（化学型1）和三个（化学型2）样品中分离出具有eremophilane和oplopane骨架的倍半萜类化合物。分离出两种新的oplopane衍生物并确定了它们的结构。在 18 个样品中，苯丙烯类化合物是主要成分（化学型 3）。在五个样品中，既没有发现苯丙烯类化合物，也没有发现倍半萜类化合物（化学型 4）。尽管化学种类繁多，但化学型与物种鉴定的形态标准之间没有发现相关性。对进化中性 DNA 区域的分析还表明，样品没有分成不同的进化枝，并且基因渗入是广泛的。
The chemical constituents of the root extracts and the evolutionarily neutral DNA base sequences were studied for 28 samples of Ligularia duciformis, L. kongkalingensis, and L. nelumbifolia collected in Sichuan and Yunnan Provinces of China. The samples could be classified into four chemotypes (14). Sesquiterpenoids having eremophilane and oplopane skeletons were isolated from two (Chemotype 1) and three (Chemotype 2) samples, respectively. Two new oplopane derivatives were isolated and their structures were determined. In 18 samples, phenylpropenoids were the major components (Chemotype 3). In five samples, neither phenylpropenoids nor sesquiterpenoids were found (Chemotype 4). Despite this large chemical variety, no correlation was found between the chemotype and the morphological criteria of species identification. The analysis of the evolutionarily neutral DNA regions also indicated that the samples were not separated into distinct clades and that introgression was extensive.