Overexpression of Malonyl-CoA: ACP Transacylase in Schizochytrium sp to Improve Polyunsaturated Fatty Acid Production
Overexpression of Malonyl-CoA: ACP Transacylase in Schizochytrium sp to Improve Polyunsaturated Fatty Acid Production
复制标题
丙二酰辅酶 A 的过表达：裂殖壶菌中的 ACP 转酰基酶可提高多不饱和脂肪酸的产量
DOI：
10.1021/acs.jafc.8b01026
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发表时间：
2018-05-30
影响因子：
6.1
通讯作者：
He, Ning
中科院分区：
文献类型：
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作者：
Li, Zhipeng;Meng, Tong;He, Ning
多不饱和脂肪酸（PUFAs）在食品和医疗工业中有着广泛的应用。本研究通过同源重组过表达丙二酰辅酶a: ACP转酰基酶（MAT），提高裂鱼PUFA的产量。结果表明，过表达MAT后，脂质和PUFA浓度分别提高了10.1和24.5%。代谢组学分析表明，工程菌株胞内三羧酸循环减弱，葡萄糖吸收加快。在甲羟戊酸途径中，细胞内胡萝卜素含量降低，然后碳通量被重定向到PUFA合成。此外，最后进行了葡萄糖补料分批发酵与工程裂壁菌。总脂质产量达到110.5 g/L，比野生菌株提高39.6%。二十二碳六烯酸和二十碳五烯酸的产率分别提高了81.5%和172.5%，达到47.39 g/L和1.65 g/L。本研究为工业PUFA生产提供了一种有效的代谢工程策略。
Polyunsaturated fatty acids (PUFAs) have been widely applied in the food and medical industry. In this study, malonyl-CoA: ACP transacylase (MAT) was overexpressed through homologous recombination to improve PUFA production in Schizochytrium. The results showed that the lipid and PUFA concentration were increased by 10.1 and 24.5% with MAT overexpression, respectively. Metabolomics analysis revealed that the intracellular tricarboxylic acid cycle was weakened and glucose absorption was accelerated in the engineered strain. In the mevalonate pathway, intracellular carotene content was decreased, and the carbon flux was then redirected toward PUFA synthesis. Furthermore, a glucose fed-batch fermentation was finally performed with the engineered Schizochytrium. The total lipid yield was further increased to 110.5 g/L, 39.6% higher than the wild strain. Docosahexaenoic acid and eicosapentaenoic acid yield were enhanced to 47.39 g/L and 1.65 g/L with an increase of 81.5 and 172.5%, respectively. This study provided an effective metabolic engineering strategy for industrial PUFA production.