Surface engineering of cardiovascular stent with endothelial cell selectivity for in vivo re-endothelialisation
Surface engineering of cardiovascular stent with endothelial cell selectivity for in vivo re-endothelialisation
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DOI：
10.1016/j.biomaterials.2012.12.036
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发表时间：
2013-04-01
期刊：
影响因子：
14
通讯作者：
Ji, Jian
中科院分区：
文献类型：
--
作者：
Wei, Yu;Ji, Ying;Ji, Jian
材料的体内内皮化为心血管植入的快速再内皮化提供了一种有前途的策略。尽管许多研究都集中在通过固定化生物活性分子来提高快速内皮化，但应该注意的是，内皮细胞（ECs）在体内会与其他类型的细胞竞争。因此，在不考虑细胞竞争的情况下部分促进EC生长的努力可能具有误导性，并且在体内没有意义。在这项研究中，我们证明了人脐静脉内皮细胞（HUVECs）对人主动脉平滑肌细胞（HASMCs）的竞争生长可以通过对磷胆碱的非特异性抗性和对REDV肽的特异性识别的协同作用而增加。进一步的体内数据表明，内皮细胞对SMCs的竞争能力，而不是内皮细胞的数量，是体内形成纯内皮层从而获得更好的抗再狭窄效果的更重要的标准。因此，支架的表面定制以获得高内皮细胞选择性可能是原位内皮化的有效设计标准，并且可能是解决支架内再狭窄问题的未来解决方案。(c) 2013 Elsevier Ltd.版权所有。
The in vivo endothelialisation of materials provides a promising strategy for the rapid re-endothelialisation of a cardiovascular implantation. Although many studies have focused on improving the rapid endothelialisation through the immobilisation of bioactive molecules, it should be noted that the endothelial cells (ECs) will compete with other cell types in vivo. Thus, the efforts to partially enhance the EC growth without considering the cell competition might be misleading and meaningless in vivo. In this study, we demonstrated that the competitive growth of human umbilical vein endothelial cells (HUVECs) over human aortic smooth muscle cells (HASMCs) could be increased through the synergic action of the nonspecific resistance to phosphorylcholine and the specific recognition of the REDV peptide. Further in vivo data indicate that the competitive ability of ECs over SMCs, instead of the number of ECs, is a significantly more important criterion for the development of a pure endothelial layer in vivo and thus the attainment of a better anti-restenosis effect. Consequently, the surface tailoring of a stent to obtain high endothelial cell selectivity is likely an effective design criterion for in situ endothelialisation and a possible future solution for the problem of in-stent restenosis. (c) 2013 Elsevier Ltd. All rights reserved.