Expression of the prolactin gene in human decidua-chorion.
Expression of the prolactin gene in human decidua-chorion.
复制标题
催乳素基因在人蜕膜绒毛膜中的表达。
DOI：
10.1210/endo-112-3-1133
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发表时间：
1983
期刊：
影响因子：
4.8
通讯作者：
J. Funder
中科院分区：
文献类型：
--
作者：
J. Clements;P. Whitfeld;N. Cooke;D. Healy;B. Matheson;J. Shine;J. Funder
从人蜕膜绒毛膜中纯化的信使RNA （mRNA）与人泌乳素32P-cDNA探针杂交。相比之下，来自人滋养细胞和羊膜的mRNA不能与催乳素cDNA杂交。人蜕膜绒毛膜催乳素特异性mRNA在琼脂糖凝胶上的迁移位置与羊垂体催乳素特异性mRNA的迁移位置相似，提示催乳素mRNA编码在这些不同的组织和物种中具有相似的大小。这些数据明确地表明，催乳素基因在人蜕膜绒毛膜中表达，证实了以前的免疫化学报道，该组织在培养中产生催乳素。
Messenger RNA (mRNA) purified from human decidua-chorion hybridizes with a 32P-cDNA probe for human prolactin. In contrast, mRNA from human trophoblast and amnion does not hybridize to prolactin cDNA. The migration position on agarose gels of prolactin-specific mRNA from human decidua-chorion is similar to that for mRNA from ovine pituitary, suggesting a similar sized mRNA coding for prolactin in these different tissues and species. These data demonstrate unambiguously that the prolactin gene is expressed in human decidua-chorion, confirming previous immunochemical reports of prolactin production by this tissue in culture.