DIVERGENT TRANSCRIPTION OF EARLY 35-KILODALTON AND 94-KILODALTON PROTEIN GENES ENCODED BY THE HINDIII K-GENOME FRAGMENT OF THE BACULOVIRUS AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS
DIVERGENT TRANSCRIPTION OF EARLY 35-KILODALTON AND 94-KILODALTON PROTEIN GENES ENCODED BY THE HINDIII K-GENOME FRAGMENT OF THE BACULOVIRUS AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS
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DOI：
10.1128/jvi.61.7.2264-2272.1987
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发表时间：
1987-07-01
影响因子：
5.4
通讯作者：
MILLER, LK
中科院分区：
文献类型：
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作者：
FRIESEN, PD;MILLER, LK
通过结合核苷酸测序、转录作图和杂交选择的 RNA 的体外翻译，确定了苜蓿银纹夜蛾核多角体病毒基因组 3.7 千碱基对 HindIII-K/EcoRI-S 区域内病毒基因的组织（85 至 88 个图谱单位）。鉴定出两个不重叠的基因，它们以相反的方向延伸并编码分子量为35,000和94,000的多肽（35K和94K多肽）。感染后早期（2 小时），两个基因均转录出未剪接的信使活性 RNA。表明立即早期基因，不同 RNA 的转录不受蛋白质合成抑制剂放线菌酮的影响。在感染后期，丰富的RNA从位于编码35K多肽的基因上游至少2.5kb碱基对的启动子转录。这些转录物与35K和94K多肽基因完全重叠，但显然缺乏蛋白质编码潜力，表明这些转录物可能在抑制早期病毒基因表达中发挥作用。
The organization of viral genes within the 3.7-kilobase-pair HindIII-K/EcoRI-S region of the Autographa californica nuclear polyhedrosis virus genome (85 to 88 map units) was determined by using a combination of nucleotide sequencing, transcriptional mapping, and in vitro translation of hybrid selected RNA. Two nonoverlapping genes, extending in opposite directions and encoding polypeptides with molecular weights of 35,000 and 94,000 (35K and 94K polypeptides), were identified. Unspliced, messenger-active RNAs were transcribed from both genes early (2 h) after infection. Indicative of immediate-early genes, transcription of the divergent RNAs was unaffected by the protein synthesis inhibitor, cycloheximide. Late in infection, abundant RNAs were transcribed from promoters located at least 2.5 kilobase pairs upstream from the gene encoding the 35K polypeptide. These transcripts completely overlapped both the 35K and 94K polypeptide genes but apparently lacked protein-coding potential, suggesting that the transcripts may play a role in the suppression of early viral gene expression.