Effects of Lissoclibadins and Lissoclinotoxins, Isolated from a Tropical Ascidian Lissoclinum cf. badium, on IL-8 production in a PMA-stimulated Promyelocytic Leukemia Cell Line
Effects of Lissoclibadins and Lissoclinotoxins, Isolated from a Tropical Ascidian Lissoclinum cf. badium, on IL-8 production in a PMA-stimulated Promyelocytic Leukemia Cell Line
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DOI：
10.3390/md401015
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发表时间：
2006-02
期刊：
影响因子：
5.4
通讯作者：
T. Oda;T. Fujiwara;Hongwei Liu;K. Ukai;R. E. Mangindaan;M. Mochizuki;M. Namikoshi
中科院分区：
文献类型：
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作者：
T. Oda;T. Fujiwara;Hongwei Liu;K. Ukai;R. E. Mangindaan;M. Mochizuki;M. Namikoshi
从热带海鞘Lissoclinum cf.检测了badium对PMA刺激的HL-60细胞产生IL-8的影响。Lissoclibadins 2（2）和3（3）以及Lissoclinotoxin F（5）以剂量依赖性方式增加IL-8的产生。化合物2和5分别是具有反式和顺式取向的二聚体结构的结构异构体，并且在诱导IL-8水平上显示出非常相似的活性。化合物3和Lissoclinotoxin E（4）也是分别具有二聚体反式和顺式结构的结构异构体，但4不诱导IL-8的产生。利索克利巴定1（1，三聚体化合物）和两种单体化合物（6和7）不增加IL-8水平。因此，它们的结构差异显著影响IL-8的产生活性、细胞增殖抑制和HL-60细胞的存活。Lissoclibadin 2是本研究中测试的七种代谢物中最有趣的化合物。
The effects of seven compounds 1–7, isolated from a tropical ascidian Lissoclinum cf. badium, on IL-8 production in PMA-stimulated HL-60 cells were examined. Lissoclibadins 2 (2) and 3 (3) and lissoclinotoxin F (5) increased the IL-8 production in a dose-dependent manner. Compounds 2 and 5 are structural isomers possessing dimeric structures of trans and cis-orientations, respectively, and showed a very similar activity on the induction of IL-8 levels. Compound 3 and lissoclinotoxin E (4) are also structural isomers having dimeric trans and cis-structures, respectively, but 4 did not induce the IL-8 production. Lissoclibadin 1 (1, trimeric compound) and two monomeric compounds (6 and 7) did not increase the IL-8 level. Therefore, the differences in their structures remarkably affected the IL-8 production activity, the inhibition of cell proliferation, and the survival of HL-60 cells. Lissoclibadin 2 was the most interesting compound of the seven metabolites tested in this study.