Stem Cells Derived from Human Amniotic Fluid Contribute to Acute Kidney Injury Recovery
Stem Cells Derived from Human Amniotic Fluid Contribute to Acute Kidney Injury Recovery
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DOI：
10.2353/ajpath.2010.091245
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发表时间：
2010-10-01
影响因子：
6
通讯作者：
Camussi, Giovanni
中科院分区：
文献类型：
--
作者：
Hauser, Peter V.;De Fazio, Roberta;Camussi, Giovanni
从人类羊水中分离的干细胞因其治疗潜力而受到关注。在这项工作中，我们研究了这些细胞是否有助于实验性急性肾损伤后的肾小管再生。从人羊水中分离表达具有多分化潜能的干细胞标志物的细胞。比较人羊水干细胞与骨髓间充质干细胞的再生潜能。我们发现，与注射间充质干细胞相比，静脉注射3.5 × 10（5）人羊水干细胞到患有甘油诱导的急性肾损伤的非免疫活性小鼠中，随后肾功能迅速恢复正常。两种干细胞类型均显示出增强的肾小管细胞增殖和减少的凋亡。间充质干细胞比羊水来源的干细胞更有效地诱导增殖，相反，羊水来源的干细胞更抗凋亡。这两种细胞类型被发现在肾小管周围毛细血管和输卵管内积累，但羊水干细胞比间充质干细胞更持久。体外实验表明，这两种细胞类型产生不同的细胞因子和生长因子，这表明不同介质的组合参与了它们的生物学作用。这些结果表明，羊水来源的干细胞可以促进急性肾损伤的肾再生，但它们并不比间充质干细胞更有效。(Am J Pathol 2010，177：2011-2021; DOE 10.2353/ajpath.2010.091245）
Stem cells isolated from human amniotic fluid are gaining attention with regard to their therapeutic potential. In this work, we investigated whether these cells contribute to tubular regeneration after experimental acute kidney injury. Cells expressing stem cell markers with multidifferentiative potential were isolated from human amniotic fluid. The regenerative potential of human amniotic fluid stem cells was compared with that of bone marrow-derived human mesenchymal stem cells. We found that the intravenous injection of 3.5 x 10(5) human amniotic fluid stem cells into nonimmune-competent mice with glycerol-induced acute kidney injury was followed by rapid normalization of renal function compared with injection of mesenchymal stem cells. Both stem cell types showed enhanced tubular cell proliferation and reduced apoptosis. Mesenchymal stem cells were more efficient in inducing proliferation than amniotic fluid-derived stem cells, which, in contrast, were more antiapoptotic. Both cell types were found to accumulate within the peritubular capillaries and the interstitium, but amniotic fluid stem cells were more persistent than mesenchymal stem cells. In vitro experiments demonstrated that the two cell types produced different cytokines and growth factors, suggesting that a combination of different mediators is involved in their biological actions. These results suggest that the amniotic fluid-derived stem cells may improve renal regeneration in acute kidney Injury, but they are not more effective than mesenchymal stem cells. (Am J Pathol 2010, 177:2011-2021; DOE 10.2353/ajpath.2010.091245)