Inhibitory effect of ginsenoside Rb1 on cardiac hypertrophy induced by monocrotaline in rat
Inhibitory effect of ginsenoside Rb1 on cardiac hypertrophy induced by monocrotaline in rat
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DOI：
10.1016/j.jep.2007.01.006
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发表时间：
2007-05-22
影响因子：
5.4
通讯作者：
Wu Qin
中科院分区：
文献类型：
--
作者：
Jiang Qing-Song;Huang Xie-Nan;Wu Qin
人参，即人参的根，在中国作为治疗多种疾病的民间药物已有数千年的历史。人参的有效成分之一人参皂苷Rb-1（Rb-1）已被报道可释放一氧化氮并减少心肌细胞内的游离Ca2+，两者在抗肥厚作用中发挥重要作用。本研究旨在探讨Rb-1对野百合碱（MCT）诱导的右心室肥厚（RVH）的潜在作用及其对钙调神经磷酸酶（CaN）信号转导通路的可能影响。 MCT治疗的动物从第1天到第14天（预防性施用）或从第15天到第28天（治疗性施用）施用Rb（10和40mg/kg），或施用载体作为相应的对照。 2周后，所有MCT治疗组动物的右心室均出现明显的肥厚反应，包括RVH指数和心房利钠肽mRNA的表达（p < 0.05），Rb-1预防组和治疗组的心肌病理形态学均显着降低（p < 0.05）。同样，MCT治疗导致心肌细胞CaN、NFAT(3)和GATA(4)的mRNA和/或蛋白高表达(p < 0.05)，而Rb-1可以减轻上述这些因子的表达(p < 0.05)。这些结果表明Rb-1治疗可以抑制MCT诱导的RVH，这可能与其对CaN信号转导通路的抑制作用有关。 (c) 2007 Elsevier Ireland Ltd. 保留所有权利。
Ginseng, the root of Panax ginseng, has been used as folk medicine in the treatment of various diseases for thousands of years in China. Ginsenoside Rb-1 (Rb-1), one of the effective components of ginseng, has been reported to release nitric oxide and decrease intracellular free Ca2+ in cardiac myocytes, both of which play important roles in antihypertrophic effect. This study was to investigate the potential effect of Rb-1 on right ventricular hypertrophy (RVH) induced by monocrotaline (MCT) and its possible influence on calcineurin (CaN) signal trasnsduction pathway. MCT-treated animals were administered with Rb, (10 and 40 mg/kg) from day 1 to day 14 (preventive administration) or from day 15 to day 28 (therapeutic administration), or with vehicle as corresponding controls. After 2 weeks, significantly hypertrophic reactions, including RVH index and the expressions of atrial natriuretic peptide mRNA, appeared in right ventricle of all MCT-treated animals (p < 0.05), which were significantly decreased with some improvements of myocardial pathomorphology in both Rb-1 prevention- and therapy-groups (p < 0.05). Similarly, MCT-treatment caused the high expressions of mRNA and/or proteins of CaN, NFAT(3) and GATA(4) from cardiocytes (p < 0.05) and Rb-1 could alleviate the expressions of these factors above (p < 0.05). These results suggest that Rb-1 treatment can inhibit the RVH induced by MCT, which may be involved in its inhibitory effects on CaN signal transduction pathway. (c) 2007 Elsevier Ireland Ltd. All rights reserved.