REVERSIBLE PACHYTENE ARREST OF SACCHAROMYCES-CEREVISIAE AT ELEVATED-TEMPERATURE
REVERSIBLE PACHYTENE ARREST OF SACCHAROMYCES-CEREVISIAE AT ELEVATED-TEMPERATURE
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DOI：
10.1007/bf00384382
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
GOETSCH, L
中科院分区：
文献类型：
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作者：
BYERS, B;GOETSCH, L
研究了一种缺乏已知温度敏感基因的酵母菌株的产孢温度敏感性。电镜细胞学观察表明，在抑制产孢温度的产孢培养基中培养的细胞在减数分裂前期被抑制。通过存在重复（但未分离）的纺锤极体和突触复合体，确定了阻滞阶段为粗线期。将冻结的培养物转移到较低的温度，使减数分裂和产孢恢复；转移到营养培养基导致有丝分裂的恢复。对恢复有丝分裂的细胞的遗传分析显示，在逮捕时，基因内重组的承诺已经发生。在高温下的长时间孵育导致基因内重组高于正常水平，这表明重组的某些方面在粗线素停止期间继续发生。证据表明，DNA复制，虽然在总体上抑制了培养，发生完成在许多被捕的细胞。
The temperature sensitivity of sporulation in a well-characterized yeast strain lacking any known temperature sensitive genes was investigated. Cytological observations by EM demonstrate that cells incubated in sporulation medium at a temperature inhibitory to sporulation became arrested in meiotic prophase. The stage of arrest was identified as a pachytene by the presence of duplicated (but unseparated) spindle pole bodies and synaptonemal complex. Transfer of the arrested culture to lower temperature permitted resumption of meiosis and sporulation; transfer to vegetative medium resulted in reversion to mitotic division. Genetic analysis of cells that had reverted to mitosis revealed that commitment to intragenic recombination had occurred by the time of arrest. Prolonged incubation at the elevated temperature resulted in the enhancement of intragenic recombination above normal levels, suggesting that some aspect of recombination continued to occur during the pachytene arrest. Evidence is presented that DNA replication, although depressed overall in the arrested cultures, had occurred to completion in many arrested cells.