THE EXPRESSION OF TUMOR-REJECTION ANTIGEN MAGE GENES IN HUMAN GASTRIC-CARCINOMA
THE EXPRESSION OF TUMOR-REJECTION ANTIGEN MAGE GENES IN HUMAN GASTRIC-CARCINOMA
复制标题
DOI：
10.1016/0016-5085(95)90639-8
复制
发表时间：
1995-11-01
期刊：
影响因子：
29.4
通讯作者：
AKIYOSHI, T
中科院分区：
文献类型：
--
作者：
INOUE, H;MORI, M;AKIYOSHI, T
背景与目的：MAGE-1和MAGE-3基因编码黑色素瘤肽抗原，可被主要组织相容性复合体限制性细胞毒性T淋巴细胞识别。这些抗原可能是免疫治疗的靶点。然而，关于这些基因在胃癌中的表达的信息很少。因此，我们对胃癌中MAGE基因的表达进行了评价。方法：采用逆转录聚合酶链反应法研究MAGE-1、MAGE-2、MAGE-3基因在肿瘤及相应正常组织标本中的表达。根据肿瘤的临床病理因素对结果进行分析。结果：在研究的68例胃癌中，分别有41%、31%和38%的患者检测到MAGE-1、MAGE-2和MAGE-3信使RNA。50%的胃癌至少表达一种MAGE基因，正常胃组织中未检测到这三种MAGE蛋白的信使RNA，胃癌中MAGE基因的表达与肿瘤的临床病理无显著相关性。结论：MAGE-1、MAGE-2和MAGE-3在胃癌中表达的比例很高，这些肿瘤排斥抗原可能为免疫治疗提供肿瘤特异性靶点。
Background & Aims: The genes MAGE-1 and MAGE-3 both encode melanoma peptide antigens recognized by major histocompatibility complex-restricted cytotoxic T lymphocytes. The antigens may be a target for immunotherapy. There is, however, little information on the expression of these genes in gastric carcinomas. Therefore, the expression of MAGE genes in gastric carcinomas was evaluated. Methods: The expression of MAGE-1, MAGE-2, and MAGE-3 genes in tumors and corresponding normal tissue specimens was studied using a reverse-transcription polymerase chain reaction. The results were analyzed according to clinicopathologic factors of the tumor. Results: In the 68 gastric carcinomas studied, MAGE-1, MAGE-2, and MAGE-3 messenger RNA were detected in 41%, 31%, and 38%, respectively. Fifty percent of the gastric carcinomas expressed at least one of the MAGE genes, Messenger RNA for the three MAGE proteins was not detected in normal gastric tissue, MAGE gene expression in gastric carcinomas was not associated with a significant clincopathology of the tumor. However, gene expression was lower in mucinous carcinomas (3 of 10), Conclusions: MAGE-1, MAGE-2, and MAGE-3 are expressed in a high percentage of gastric carcinomas, These tumor rejection antigens may provide tumor-specific targets for immunotherapy,