Occurrence of Plasmids in the Aromatic Degrading Bacterioplankton of the Baltic Sea
Occurrence of Plasmids in the Aromatic Degrading Bacterioplankton of the Baltic Sea
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DOI：
10.3390/genes2040853
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发表时间：
2011-12-01
期刊：
影响因子：
3.5
通讯作者：
Heinaru, Ain
中科院分区：
文献类型：
--
作者：
Jutkina, Jekaterina;Heinaru, Eeva;Heinaru, Ain
质粒是一种可移动的遗传元件，为宿主提供了许多有益的特征，包括在某些情况下能够降解不同的芳香化合物。为了填补关于波罗的海海水分解质粒的知识空白，总共分离并筛选了209株生物降解菌，以确定是否存在这些可移动的遗传元件。我们发现，在可培养的波罗的海浮游细菌中，大小质粒都很常见，尤其是在细菌属假单胞菌和不动杆菌中。在61株含质粒菌株中，有34株携带大质粒，这可能与宿主菌的生物降解能力有关。针对IncP-9表达载体的多样性，检测到了自传性间甲苯酸(TOL)和水杨酸(SAL)表达载体。对携带IncP-9的分离株的REPA基因进行了测序，显示了IncP-9质粒家族内的高度多样性，并扩大了IncP-9代表的细菌宿主种类的假设范围。这项研究首次深入了解了波罗的海浮游细菌中INCP-9分解代谢质粒的基因库。
Plasmids are mobile genetic elements that provide their hosts with many beneficial traits including in some cases the ability to degrade different aromatic compounds. To fulfill the knowledge gap regarding catabolic plasmids of the Baltic Sea water, a total of 209 biodegrading bacterial strains were isolated and screened for the presence of these mobile genetic elements. We found that both large and small plasmids are common in the cultivable Baltic Sea bacterioplankton and are particularly prevalent among bacterial genera Pseudomonas and Acinetobacter. Out of 61 plasmid-containing strains (29% of all isolates), 34 strains were found to carry large plasmids, which could be associated with the biodegradative capabilities of the host bacterial strains. Focusing on the diversity of IncP-9 plasmids, self-transmissible m-toluate (TOL) and salicylate (SAL) plasmids were detected. Sequencing the repA gene of IncP-9 carrying isolates revealed a high diversity within IncP-9 plasmid family, as well as extended the assumed bacterial host species range of the IncP-9 representatives. This study is the first insight into the genetic pool of the IncP-9 catabolic plasmids in the Baltic Sea bacterioplankton.