Design and characterization of programmable DNA nanotubes
Design and characterization of programmable DNA nanotubes
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DOI：
10.1021/ja044319l
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发表时间：
2004-12-22
影响因子：
15
通讯作者：
Winfree, E
中科院分区：
文献类型：
--
作者：
Rothemund, PWK;Ekani-Nkodo, A;Winfree, E
DNA自组装为从纳米级组分合成复杂结构提供了一种可编程的自下而上的方法。尽管纳米管是基于瓦片的DNA自组装中出现的一种基本形式，但控制管结构的因素仍知之甚少。在此我们报道并描述了一种由DNA双交叉分子（DAE - E瓦片）制成的新型纳米管。未修饰的纳米管直径在7到20纳米之间（周长为4到10个瓦片），可生长至50微米长，持续长度约为4微米，并且可以通过编程显示多种图案。对修饰的研究（1）证实了粘性末端堆叠的重要性，（2）确认了纳米管内外表面的特性，（3）确定了对纳米管尺寸和形态有深远影响的瓦片特征。基于这些结果，纳米管结构可以通过一个基于B型DNA的几何形状和能量学的简单模型来解释。
DNA self-assembly provides a programmable bottom-up approach for the synthesis of complex structures from nanoscale components. Although nanotubes are a fundamental form encountered in tile-based DNA self-assembly, the factors governing tube structure remain poorly understood. Here we report and characterize a new type of nanotube made from DNA double-crossover molecules (DAE-E tiles). Unmodified tubes range from 7 to 20 nm in diameter (4 to 10 tiles in circumference), grow as long as 50 mum with a persistence length of similar to4 mum, and can be programmed to display a variety of patterns. A survey of modifications (1) confirms the importance of sticky-end stacking, (2) confirms the identity of the inside and outside faces of the tubes, and (3) identifies features of the tiles that profoundly affect the size and morphology of the tubes. Supported by these results, nanotube structure is explained by a simple model based on the geometry and energetics of B-form DNA.