Different neuronal populations of the rat median preoptic nucleus express c-fos during sleep and in response to hypertonic saline or angiotensin-II.
Different neuronal populations of the rat median preoptic nucleus express c-fos during sleep and in response to hypertonic saline or angiotensin-II.
复制标题
大鼠正中视前核的不同神经元群在睡眠期间以及对高渗盐水或血管紧张素-II 的反应中表达 c-fos。
DOI：
10.1113/jphysiol.2005.097212
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Szymusiak,R
中科院分区：
文献类型：
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作者：
Gvilia,I;Angara,C;McGinty,D;Szymusiak,R
下丘脑的正中视前核（MnPN）含有睡眠活性神经元，该核中睡眠相关的Fos免疫反应性（IR）主要在gaba能细胞中表达。MnPN还包含对高渗盐水和血管紧张素- II （Ang - II）有反应的细胞。为了阐明参与睡眠和体液平衡调节的MnPN神经元之间的功能关系，我们在自然睡眠和睡眠剥夺的大鼠中检测了高渗盐水和Ang - II后MnPN中c - fos的表达。与对照动物相比，在自发睡眠和睡眠剥夺的大鼠中，全身给药高渗盐水和脑室内注射Ang - II可增加Fos - IR。为了确定在渗透和激素刺激下激活的MnPN神经元数量是否与睡眠时激活的神经元相似或不同，我们量化了自发睡眠高渗盐水和Ang - II处理的大鼠与各自的对照大鼠中MnPN gaba能神经元中的Fos - IR。这些治疗引起的Fos - IR主要发生在非gaba能神经元中（80-85%）。本研究的发现提供了单独的MnPN神经元群参与睡眠和体液稳态调节的证据。
The median preoptic nucleus (MnPN) of the hypothalamus contains sleep‐active neurones, and sleep‐related Fos‐immunoreactivity (IR) in this nucleus is primarily expressed in GABAergic cells. The MnPN also contains cells responsive to hypertonic saline and to angiotensin‐II (Ang‐II). To clarify functional relationships between MnPN neurones involved in the regulation of sleep and body fluid homeostasis, we examined c‐fosexpression in the MnPN after administration of hypertonic saline and Ang‐II in both spontaneously sleeping and sleep‐deprived rats. Systemic administration of hypertonic saline and intracerebroventricular (i.c.v.) injection of Ang‐II increased Fos‐IR in both spontaneously sleeping and sleep‐deprived rats, compared to control animals. To determine if the population of MnPN neurones activated in response to osmotic and hormonal stimuli is similar to or different from neurones activated during sleep, we quantified Fos‐IR in MnPN GABAergic neurones in spontaneously sleeping hypertonic saline‐ and Ang‐II‐treated ratsversusrespective control rats. Fos‐IR evoked by these treatments occurred primarily (80–85%) in non‐GABAergic neurones. Findings of the present study provide evidence that separate populations of MnPN neurones are involved in the regulation of sleep and body fluid homeostasis.