Avoidance of Heat and Attraction to Optogenetically Induced Sugar Sensation as Operant Behavior in Adult Drosophila
Avoidance of Heat and Attraction to Optogenetically Induced Sugar Sensation as Operant Behavior in Adult Drosophila
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DOI：
10.3109/01677063.2012.700266
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发表时间：
2012-09
影响因子：
1.9
通讯作者：
Nidhi Nuwal;Patrick Stock;Jochen Hiemeyer;Benjamin Schmid;A. Fiala;E. Buchner
中科院分区：
文献类型：
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作者：
Nidhi Nuwal;Patrick Stock;Jochen Hiemeyer;Benjamin Schmid;A. Fiala;E. Buchner
动物必须根据内部状态和环境状况进行适当的行为动作，并根据积极或消极的后果调整其行为。果蝇是研究适应性行为神经机制的重要模式生物。作者正在使用一种行为范例，其中连接到操纵器的果蝇可以在聚苯乙烯泡沫塑料球上行走，该聚苯乙烯泡沫塑料球的运动被记录，从而可以记录苍蝇的预期左转或右转，并用于操作性地控制热刺激或不同神经元子集的光遗传激活。作为原理的证明，作者发现，在这种情况下，苍蝇避免热刺激，但更喜欢糖受体的光遗传学自我刺激。使用这种设置，现在应该有可能研究的神经网络的积极和消极的价值评估的成年果蝇在操作设置。
Animals have to perform adequate behavioral actions dependent on internal states and environmental situations, and adjust their behavior according to positive or negative consequences. The fruit fly Drosophila melanogaster represents a key model organism for the investigation of neuronal mechanisms underlying adaptive behavior. The authors are using a behavioral paradigm in which fruit flies attached to a manipulator can walk on a Styrofoam ball whose movements are recorded such that intended left or right turns of the flies can be registered and used to operantly control heat stimuli or optogenetic activation of distinct subsets of neurons. As proof of principle, the authors find that flies in this situation avoid heat stimuli but prefer optogenetic self-stimulation of sugar receptors. Using this setup it now should be possible to study the neuronal network underlying positive and negative value assessment of adult Drosophila in an operant setting.