Growth characteristics of respiratory syncytial virus.
Growth characteristics of respiratory syncytial virus.
复制标题
呼吸道合胞病毒的生长特性。
DOI：
10.4049/jimmunol.88.5.581
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发表时间：
1962
影响因子：
4.4
通讯作者：
W. S. Jordan
中科院分区：
文献类型：
--
作者：
W. S. Jordan
本文研究了呼吸道合胞病毒在组织培养中的细胞类型和营养液成分对细胞病变和增殖的影响。合胞体的产生在HEp-2细胞中是显著的，在KB和HeLa细胞中是最小的;变圆是DMB细胞的特征性反应。与含有Eagle最小必需培养基的维持液相比，使用谢勒溶液的维持液限制了合胞体的形成并延迟了细胞病变效应的发展，特别是在HEp-2细胞中。合胞体的存在与否对感染性病毒的总产量影响不大。
初始增殖周期的潜伏期为12 ~ 14小时，随后迅速生长并释放病毒。使用一种释放细胞相关病毒而几乎不灭活的细胞破碎方法，证明即使在细胞病变效应显著时，近50%的病毒仍与细胞相关。一些病毒通过单次冻融循环灭活，但在-70 ° C下储存的等分试样保持稳定和足够水平的感染性病毒至少6个月。
Respiratory syncytial virus was studied with respect to the effect of type of cell and composition of nutrient fluid on cytopathic manifestations and multiplication in tissue culture. Production of syncytium was striking in HEp-2 cells, minimal in KB and HeLa cells; rounding was the characteristic response of DMB cells. In comparison with a maintenance fluid containing Eagle's minimal essential medium, a fluid utilizing Scherer's solution limited the formation of syncytium and delayed the evolution of cytopathic effect, particularly in HEp-2 cells. The presence or absence of syncytium had little effect on the total yield of infectious virus.
The latent period of the initial multiplication cycle was 12 to 14 hr, and was followed by rapid growth and release of virus. A method for cell disruption which released cell-associated virus with little inactivation was utilized to demonstrate that nearly 50% of virus is still cell-associated even when the cytopathic effect is marked. Some virus was inactivated by a single freeze-thaw cycle, but aliquots stored at -70°C maintained stable and adequate levels of infectious virus for at least 6 months.