Angelica sinensis polysaccharide nanoparticles as novel non-viral carriers for gene delivery to mesenchymal stem cells
Angelica sinensis polysaccharide nanoparticles as novel non-viral carriers for gene delivery to mesenchymal stem cells
复制标题
当归多糖纳米粒子作为新型非病毒载体将基因传递至间充质干细胞。
DOI：
10.1016/j.nano.2013.05.008
复制
发表时间：
2013-11-01
影响因子：
5.4
通讯作者：
Yu, Jiangnan
中科院分区：
文献类型：
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作者：
Deng, Wenwen;Fu, Min;Yu, Jiangnan
本研究以当归多糖纳米粒为载体，构建了一种高效、安全的非病毒基因载体。在用支链低分子量聚乙烯亚胺（1200 Da）修饰后，将阳离子化ASP（cASP）与编码转化生长因子-β 1（TGF-β 1）的质粒组合以形成球形纳米级颗粒（即，cASP-pTGF-β 1纳米颗粒）。将该纳米颗粒用于转染大鼠骨髓间充质干细胞和人脐带间充质干细胞。结果，纳米颗粒（cASP/pDNA重量比10：1）在两种细胞中具有最大的转染效率，其显著高于Lipofectamine 2000和PEI（25 kDa）的转染效率。这与半定量RT-PCR和活细胞成像的结果一致。这些纳米颗粒的毒性也低于Lipofectamine 2000和PEI（25 kDa）。因此，cASP可能是一种新型的非病毒基因vector.From临床编辑的潜在候选人：这些作者证明了通过基于当归多糖的球形纳米颗粒使用基于纳米颗粒的有效和安全的非病毒基因载体递送系统，其参数上级于Lipofectamine 2000。(C)2013 Elsevier Inc. All rights reserved.
This study centers on the use of a nanoparticle based on the polysaccharide from Angelica sinensis (ASP) as an efficient and safe non-viral gene vector. After modification with branched low molecular weight polyethylenimine (1200 Da), the cationized ASP (cASP) was combined with the plasmid encoding transforming growth factor-beta 1 (TGF-beta 1) to form a spherical nano-scaled particle (i.e., cASP-pTGF-beta 1 nanoparticle). This nanoparticle was applied to transfect rat bone marrow mesenchymal stem cells and human umbilical cord mesenchymal stem cells. As a result, nanoparticles (cASP/pDNA weight ratio 10: 1) had the greatest transfection efficiency in both cells, which was significantly higher than those of Lipofectamine2000 and PEI (25 kDa). This was in agreement with the findings of the semi-quantitative RT-PCR and live cell imaging. These nanoparticles were also less toxic than Lipofectamine2000 and PEI (25 kDa). Therefore, cASP could be a potential candidate for a novel non-viral gene vector.From the Clinical Editor: These authors demonstrate the use of a nanoparticle-based efficient and safe non-viral gene vector delivery system via a spherical nanoparticle based on a polysaccharide from Angelica sinensis, with parameters superior to Lipofectamine2000. (C) 2013 Elsevier Inc. All rights reserved.