Acute and chronic toxicity of a polyherbal preparation - Jueyin granules.
Acute and chronic toxicity of a polyherbal preparation - Jueyin granules.
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一种中药制剂——厥阴颗粒的急、慢性毒性
DOI：
10.1186/s12906-018-2211-z
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发表时间：
2018-05-08
影响因子：
--
通讯作者：
Li FL
中科院分区：
文献类型：
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作者：
Chen Y;Guo DJ;Deng H;Wu MF;Zhang YN;Li S;Xu R;Chen J;Jin XX;Li B;Xu Q;Li FL
近年来，中草药的潜在毒性越来越引起人们的关注。决银颗粒(JYG)是一种中药配方，在动物模型和临床研究中都被证明是治疗银屑病的有效药物。然而，对JYG可能的急性和慢性毒性知之甚少。本研究的目的是观察健银颗粒对ICR小鼠和Wistar大鼠的安全性。为考察健肾颗粒的急性毒性，将ICR小鼠随机分为实验组和对照组，每组20只，雌雄各10只。试验组按21.5 mg/kg剂量，相当于临床人体剂量的143倍，连续14d灌胃，对照组喂以蒸馏水。在慢性毒性试验中，将Wistar大鼠分为4组，每组40只，雄性和雌性各20只。实验动物分别给予JYG 7.5g/kg、3.75g/kg和1.875 mg/kg灌胃，对照组给予蒸馏水灌胃。每周都会监测这些动物的体重、食物和水的消耗量。此外，在特定的观察时间点测量它们的生化和血液学参数、组织病理学以及体重和器官重量。急性毒性试验结果显示，健脾颗粒组小鼠未见死亡，主要脏器未见异常病理改变。在慢性毒性试验中，健肝颗粒未引起大鼠生理参数的明显异常或主要器官的病理改变。结果表明，JYG在一定剂量下对动物无任何不良影响。因此，JYG在所研究的剂量水平下是安全的，并且在动物模型中没有引起急性或慢性毒性。本文的在线版本(10.1186/s12906-0182211-z)包含补充材料，可供授权用户使用。
The potential toxicity of Chinese herbal medicine has attracted more attention in recent years. Jueyin granules (JYG), a polyherbal formula, have been proven to be an effective agent for treating psoriasis in both animal models and clinical research. However, little is known about the possible acute and chronic toxicity of JYG. The objective of this study was to investigate the safety of JYG in ICR mice and Wistar rats. To examine the acute toxicity of JYG, ICR mice were randomly divided into an experimental group and a control group, each comprising 20 mice (10 male and 10 female). The experimental group was fed JYG solution at a dose of 21.5 g/kg, equivalent to 143 times the clinical human dosage, for 14 days, whereas control animals were fed distilled water. In the chronic toxicity test, Wistar rats were divided into four groups, each comprising 40 rats (20 male and 20 female). For 6 months, the experimental animals were given JYG at a dose of 7.5, 3.75 and 1.875 g/kg, whereas control animals were given distilled water. The animals’ body weight, food and water consumptions were monitored weekly. In addition, their biochemical and hematological parameters, histopathology, and body and organ weights were all measured at specific observation time points. According to the results of the acute toxicity test, no mortality was found and no abnormal pathological changes in major organs were observed in mice treated with JYG. In the chronic toxicity test, JYG did not cause significant abnormalities in the physiological parameters or pathological changes in the major organs of the rats. The results indicated that JYG at the given doses did not induce any harmful effects in animals. Thus, it is reasonable to conclude that JYG is safe at the studied dosage levels and causes no acute or chronic toxicity in animal models. The online version of this article (10.1186/s12906-018-2211-z) contains supplementary material, which is available to authorized users.