EXCHANGE OF CONDUCTION PATHWAYS BETWEEN 2 RELATED K+ CHANNELS
EXCHANGE OF CONDUCTION PATHWAYS BETWEEN 2 RELATED K+ CHANNELS
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DOI：
10.1126/science.2000495
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发表时间：
1991-02-22
期刊：
影响因子：
56.9
通讯作者：
BROWN, AM
中科院分区：
文献类型：
--
作者：
HARTMANN, HA;KIRSCH, GE;BROWN, AM
离子传导途径或电压门控离子通道孔的结构尚不清楚，尽管跨膜段S5和S6之间的连接体已被认为形成钾通道中孔的一部分。 为了测试该区域是否控制钾通道传导，将S5-S6接头的21个氨基酸片段从电压激活的钾通道NGK 2移植到另一个钾通道DRK 1，其具有非常不同的孔特性。 在所得嵌合通道中，单通道电导和外部和内部四乙基铵（TEA）离子的阻断是供体NGK 2通道的特征。 因此，这21个氨基酸的片段控制孔的基本生物物理特性，并可能形成这些钾通道的传导途径。
The structure of the ion conduction pathway or pore of voltage-gated ion channels is unknown, although the linker between the membrane spanning segments S5 and S6 has been suggested to form part of the pore in potassium channels. To test whether this region controls potassium channel conduction, a 21-amino acid segment of the S5-S6 linker was transplanted from the voltage-activated potassium channel NGK2 to another potassium channel DRK1, which has very different pore properties. In the resulting chimeric channel, the single channel conductance and blockade by external and internal tetraethylammonium (TEA) ion were characteristic of the donor NGK2 channel. Thus, this 21-amino acid segment controls the essential biophysical properties of the pore and may form the conduction pathway of these potassium channels.