Metapocyrtus um sp. nov., a new weevil species (Coleoptera, Curculionidae, Entiminae, Pachyrhynchini) from Davao City, Mindanao Island, Philippines
Metapocyrtus um sp. nov., a new weevil species (Coleoptera, Curculionidae, Entiminae, Pachyrhynchini) from Davao City, Mindanao Island, Philippines
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Metapocyrtus um sp。
DOI：
10.11646/zootaxa.5068.4.10
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发表时间：
2021
期刊：
影响因子：
0.9
通讯作者：
VAN DAM, MATTHEW H.
中科院分区：
文献类型：
--
作者：
CABRAS, ANALYN A.;LAM, ATHENA W.;VAN DAM, MATTHEW H.
Metapopotus Heller 1912属是厚喙龙族中种类最多和最复杂的，有7个亚属和200多个描述的物种（Schultze，1925; Bollino et al.，2020; Cabras等人，2021年）。该属是菲律宾特有的，但由于植物或植物产品的进口，日本，马来西亚和台湾也有几个记录（Yoshitake & Tsuji，2019）。像其他Pachyrhynchini的成员一样，Metapointus融合了鞘翅，并且无法飞行，这导致了高度的特有性和狭窄的地理范围，通常局限于岛屿或山脉。在棉兰老岛大学和加州科学院进行的最后一次联合考察中，发现了一种新的Metapopotus。本文对该新种进行了描述，并对其生境作了简要描述。该物种的CO 1（细胞色素氧化酶）序列保存在NCBI数据库中。本研究所用的标本是通过薄片打浆和手工采摘收集的，并用乙酸乙酯杀死。在Luxeo 4D和Nikon SMZ 745 T体视显微镜下观察形态特征。生殖器治疗（包括阴茎内翻法）与货车Dam（2014）描述的方法相同。根据Cabras等人的研究，（2021），除了内阴茎（用65 mm微距透镜拍摄）和男性体型，其使用Zeene Stacker进行堆叠和处理。本文中提出的测量遵循Yoshitake（2011）。对于DNA制备，使用DNEasy组织试剂盒（Qiagen，Hilden-Germany）从正模的内胚周围的组织中提取DNA。PCR引物和热循环仪条件遵循Morinière et al. 2016中的条件。使用ABI-3130 xl遗传分析仪对PCR产物进行测序。使用Sequencher®版本5.4校正并重建序列。6 DNA序列分析软件，Gene Codes Corporation，安阿伯，MI USA。
The genus Metapocyrtus Heller 1912 is the most speciose and complex among the tribe Pachyrhynchini with 7 subgenera and more than 200 described species (Schultze, 1925; Bollino et al., 2020; Cabras et al., 2021). The genus is endemic to the Philippines but with several records from Japan, Malaysia and Taiwan due to importation of plants or plant products (Yoshitake & Tsuji, 2019). Like the other members of the Pachyrhynchini, Metapocyrtus have fused elytra and are incapable of flight which has resulted in high endemism and narrow geographic ranges often confined to an island or mountain range. During the last joint expedition conducted by the University of Mindanao and California Academy of Sciences, one species of Metapocyrtus was found to be new to science. In this paper, the new species is described and illustrated with a brief description of its habitat. The CO1 (cytochrome oxidase) sequence for the species is deposited in the NCBI database.The specimens examined for this study were collected through sheet beating and hand picking and killed in vials with ethyl acetate. Morphological characters were observed under Luxeo 4D and Nikon SMZ745T stereomicroscopes. The treatment of the genitalia including the method of endophallus eversion was identical to that described by Van Dam (2014). Stacked digital images were taken following Cabras et al.(2021) except for the endophallus (taken with a 65mm macro lens), and the male habitus which were stacked and processed using Zerene Stacker. Measurements presented in this paper follow Yoshitake (2011). For DNA preparation, DNA was extracted from the tissue surrounding the endophallus of the holotype using DNEasy tissue kit (Qiagen, Hilden–Germany). The PCR primers and thermocycler conditions follow those in Morinière et al. 2016. The PCR product was sequenced using an ABI-3130xl Genetic Analyzer. The sequence was corrected and reconstructed using the Sequencher® version 5.4. 6 DNA sequence analysis software, Gene Codes Corporation, Ann Arbor, MI USA.