Application of enzyme-linked immunosorbent assay for the surveillance of Mycoplasma hyopneumoniae infection in pigs.
Application of enzyme-linked immunosorbent assay for the surveillance of Mycoplasma hyopneumoniae infection in pigs.
复制标题
酶联免疫吸附试验在猪肺炎支原体感染监测中的应用。
DOI：
--
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
L. Vraa
中科院分区：
文献类型：
--
作者：
V. Sørensen;K. Barfod;Niels Christian Feld;L. Vraa
应用单克隆抗体阻断酶联免疫吸附试验（ELISA）对猪群猪支原体感染进行血清学监测。对先前针对该ELISA报告的“牛群预测值”进行了随访研究。在通过该ELISA得到阳性结果的牛群中，42%随后被发现被感染，而100%得到阴性结果的牛群未被感染。先前报告记录的阳性和阴性牛群预测值分别为39%和99.8%。在自然感染的动物中，初乳中的反应比血清中的反应更频繁，如果在分娩前或分娩后不久获得初乳样品，这种差异最为明显。咳嗽被认为是最可靠的感染临床指标，但仅通过临床畜群检查的监测未能检测到30%的感染畜群。自然暴露于M.在使用不同管理系统的两个畜群中，猪流感的情况不同：在一个畜群中，抗体在接触后三周首次检测到，而在另一个畜群中，抗体直到接触后五周才检测到。
A monoclonal antibody-based blocking enzyme-linked immunosorbent assay (ELISA) was used for serological surveillance of Mycoplasma hyopneumoniae infection in pig herds. A follow-up study was conducted on "herd predictive values" previously reported for this ELISA. Of those herds giving positive results by this ELISA, 42% were subsequently found to be infected, while 100% of herds giving negative results were uninfected. Previous reports recorded positive and negative herd predictive values of 39% and 99.8%, respectively. Among naturally-infected animals, reaction in colostrum was more frequent than in serum, and this difference was most pronounced if the colostrum samples were obtained shortly before or after farrowing. Coughing was found to be the most reliable clinical indicator of infection, but surveillance through clinical herd inspections alone failed to detect 30% of infected herds. The time required for seroconversion following natural exposure to M. hyopneumoniae differed in two herds using different management systems: in one herd antibodies were first detected three weeks post-exposure, while in the other herd antibodies were not detected until five weeks after exposure.