L-Amino acid decarboxylase- and tyrosine hydroxylase-immunoreactive cells in the extended olfactory amygdala and elsewhere in the adult prairie vole brain
L-Amino acid decarboxylase- and tyrosine hydroxylase-immunoreactive cells in the extended olfactory amygdala and elsewhere in the adult prairie vole brain
复制标题
DOI：
10.1016/j.jchemneu.2011.10.006
复制
发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Lonstein, Joseph S.
中科院分区：
文献类型：
--
作者：
Ahmed, Eman I.;Northcutt, Katharine V.;Lonstein, Joseph S.
合成多巴胺（DA）的神经元广泛分布于大脑中，并涉及大量的生理和行为功能，包括啮齿动物的社会再生产行为。最近，我们一直在调查可能参与的性别和物种特异性TH-免疫反应（TH-IR）细胞在雄性草原田鼠（Microtus ochrogaster）的主床核的纹终末（pBST）和后背内侧杏仁核（MeApd）的化学感觉控制他们的一夫一妻制pairbonding和养育行为。这些TH阳性细胞对多巴胺-β-羟化酶（DBH）没有免疫反应性，表明它们不是去甲肾上腺素能细胞，但可能是DA能细胞。DA能表型将需要他们含有芳香族L-氨基酸脱羧酶（AADC），在这里，我们研究了存在的TH和AADC的免疫反应细胞的pBST和MeApd的成年处女男性和女性草原田鼠。我们还观察了TH/AADC细胞在前腹侧室周核（AVPV）、内侧视前区（MPO）、弓状核（ARH）、齿状回（21）、黑质（SN）和腹侧被盖区（VTA）的分布。我们的发现包括：（1）pBST和MeApd各自含有完全非重叠分布的TH-1 r和AADC-1 r细胞，（2）AVPV含有令人惊讶的很少的AADC-1 r细胞，并且几乎没有TH-1 r细胞含有AADC-1 r，（3）MPO、ARH和ZI中约60%的TH-1 r细胞也含有AADC-1 r，（4）出乎意料地，SN和VTA中仅约一半的TH-1 r细胞含有AADC-1 r，（5）在传统的单胺合成区之外发现了显著的AADC-ir细胞群，包括成年实验室大鼠或猫中不含AADC-ir细胞的一些部位（内侧隔和大脑皮质）。在没有产生DA的化学要求的情况下，未交配的成年草原田鼠pBST、MeApd和它们大脑中其他地方的单酶促TH-1 R细胞可能会产生L-DOPA作为最终产物，并将其用作神经递质或神经调质，类似于在实验室大鼠大脑中观察到的单酶促TH-合成细胞。(C)2011 Elsevier B. V.保留所有权利。
Neurons synthesizing dopamine (DA) are widely distributed in the brain and implicated in a tremendous number of physiological and behavioral functions, including socioreproductive behaviors in rodents. We have recently been investigating the possible involvement of sex- and species-specific TH-immunoreactive (TH-ir) cells in the male prairie vole (Microtus ochrogaster) principal bed nucleus of the stria terminalis (pBST) and posterodorsal medial amygdala (MeApd) in the chemosensory control of their monogamous pairbonding and parenting behaviors. These TH-ir cells are not immunoreactive for dopamine-beta-hydroxylase (DBH), suggesting they are not noradrenergic but possibly DAergic. A DAergic phenotype would require them to contain aromatic L-amino acid decarboxylase (AADC) and here we examined the existence of cells immunoreactive for both TH and AADC in the pBST and MeApd of adult virgin male and female prairie voles. We also investigated the presence of TH/AADC cells in the anteroventral periventricular nucleus (AVPV), medial preoptic area (MPO), arcuate nucleus (ARH), zona incerta (21), substantia nigra (SN) and ventral tegmental area (VTA). Among our findings were: (1) the pBST and MeApd each contained completely non-overlapping distributions of TH-ir and AADC-ir cells, (2) the AVPV contained surprisingly few AADC-ir cells and almost no TH-ir cells contained AADC-ir, (3) approximately 60% of the TH-ir cells in the MPO, ARH, and ZI also contained AADC-ir, (4) unexpectedly, only about half of TH-ir cells in the SN and VTA contained AADC-ir, and (5) notable populations of AADC-ir cells were found outside traditional monoamine-synthesizing regions, including some sites that do not contain AADC-ir cells in adult laboratory rats or cats (medial septum and cerebral cortex). In the absence of the chemical requirements to produce DA, monoenzymatic TH-ir cells in the virgin adult prairie vole pBST, MeApd, and elsewhere in their brain may instead produce L-DOPA as an end product and use it as a neurotransmitter or neuromodulator, similar to what has been observed for monoenzymatic TH-synthesizing cells in the laboratory rat brain. (C) 2011 Elsevier B.V. All rights reserved.