Development and evaluation of an antigen detection dipstick assay for the diagnosis of human onchocerciasis
Development and evaluation of an antigen detection dipstick assay for the diagnosis of human onchocerciasis
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DOI：
10.1111/j.1365-3156.2004.01384.x
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Ngu, JL
中科院分区：
文献类型：
--
作者：
Ayong, LS;Tume, CB;Ngu, JL
为了提高盘尾丝虫病，特别是轻型感染的诊断，我们开发和评价了一种基于尿和泪液中旋毛虫特异性抗原检测的盘尾丝虫试纸条试验。该试验能够在样品中检测到低至25 ng/ml的寄生虫特异性抗原，并且仅需3 h。对喀麦隆盘尾丝虫病皮肤过敏地区的456名居民进行的检测评价导致408例（89.5%）尿液阳性和374例（82%）泪液阳性。盘尾丝虫病快速流行病学绘图（REMO）和皮肤切片法测定的研究地区的流行率分别为52%和36.8%。尿试纸法检测的敏感性为100%，泪液为92%，其特异性均为100%。同一个体的尿液和泪液测试结果之间的一致性为87%。当在室温下放置长达8个月时，测试条具有足够的反应性。该试验将有助于在低传播区进行盘尾丝虫病的实验室诊断，并在实验性药物研究中确定对患者的成功治疗。
To improve on the diagnosis of onchocerciasis, especially light infections, we developed and evaluated an oncho-dipstick test based on the detection of Onchocerca volvulus specific antigens in urine and tears. The test was able to detect as little as 25 ng/ml of parasite specific antigens in samples and took as little as 3 h. Evaluation of the assay on 456 residents of an onchocerciasis hyperendermic area in Cameroon resulted in 408 (89.5%) positives in urine and 374 (82%) positives in tears. The prevalence of onchocerciasis in the study area, as determined by Rapid Epidemiological Mapping of Onchocerciasis (REMO) and skin snip methods, was 52 and 36.8%, respectively. The sensitivity of the oncho-dipstick assay was 100% in urine and 92% in tears; its specificity was 100% in both. Concordance between urine and tear test results from the same individuals was 87%. The test strips were sufficiently reactive when left at room temperature for up to 8 months. The test would be useful for laboratory diagnosis of onchocerciasis in low transmission zones and to ascertain successful treatment of patients in experimental drug studies.