CHECKPOINT CONTROLS IN SCHIZOSACCHAROMYCES-POMBE - RAD1
CHECKPOINT CONTROLS IN SCHIZOSACCHAROMYCES-POMBE - RAD1
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DOI：
10.1002/j.1460-2075.1992.tb05178.x
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发表时间：
1992-04-01
期刊：
影响因子：
11.4
通讯作者：
YOUNG, PG
中科院分区：
文献类型：
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作者：
ROWLEY, R;SUBRAMANI, S;YOUNG, PG
“检查点”控制确保细胞周期的事件以有序的方式完成。例如，这样的控制延迟有丝分裂，直到DNA合成和辐射诱导的DNA损伤的修复完成。对放射敏感的裂变酵母突变体的rad系列进行了检查，以鉴定DNA损伤响应检查点控制缺陷的菌株。五人被确认。一个（rad 1 -1）和野生型的表征。rad 1 -1突变体在辐射后不停滞，对辐射杀伤敏感，并且不被羟基脲停滞，因此也缺乏DNA合成响应性检查点控制。rad 1 -1突变体的辐射敏感性大大降低时，照射和维持6小时的非分裂（密度抑制）状态，表明rad 1 -1是修复熟练和辐射敏感性，只有通过失败延迟。需要rad 1的检查点控制似乎通过cdc 2的活性来调节G2-M进程，这里通过辐射跃迁点和cdc 2执行点的重合来暗示这种作用。
'Checkpoint' controls ensure that the events of the cell cycle are completed in an orderly fashion. For example, such controls delay mitosis until DNA synthesis and repair of radiation-induced DNA damage are complete. The rad series of radiosensitive fission yeast mutants was examined to identify strains deficient for the DNA damage-responsive checkpoint control. Five were identified. A characterization of one (rad1-1) and the wild-type is presented. The rad1-1 mutant does not arrest after irradiation, is sensitive to killing by radiation and is not arrested by hydroxyurea, and thus is also deficient for the DNA synthesis-responsive checkpoint control. The radiosensitivity of the rad1-1 mutant was greatly reduced when irradiated and maintained for 6 h in a non-dividing (density inhibited) state, demonstrating that rad1-1 is repair proficient and radiosensitive only through failure to delay. The checkpoint controls for which rad1 is required appear to regulate G2-M progression through the activity of cdc2, here implicated in this role by the coincidence of the radiation transition point and the cdc2 execution point.