Antigenic and biochemical analysis of field isolates of influenza B virus: evidence for intra- and inter-epidemic variation.
Antigenic and biochemical analysis of field isolates of influenza B virus: evidence for intra- and inter-epidemic variation.
复制标题
乙型流感病毒现场分离株的抗原和生化分析：流行内和流行间变异的证据。
DOI：
10.1099/0022-1317-64-11-2367
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Schild,GC
中科院分区：
文献类型：
--
作者：
Oxford,JS;Abbo,H;Corcoran,T;Webster,RG;Smith,AJ;Grilli,EA;Schild,GC
使用一组单克隆抗体对从一所学校的流行病中分离的53株B型流感病毒的血凝素抗原进行了详细的抗原分析。可检测到13种可区分的抗原组的B型流感病毒，但其中26种病毒属于在整个流行期间共存的两组（III和IV）。抗原可区分的流感B病毒是从附近第二所学校的流行病中分离出来的。从两所学校分离的流感B病毒可以通过NS1多肽和基因1、2、3和6的不同电泳迁移率进一步区分，而来自单一学校流行的病毒在这些生化特征方面非常密切相关。这些发现与以下假设一致，即暴发是由一个人排泄抗原突变体引起的，其中主要有两种抗原突变体在流行期间传播并共存，尽管不能排除在流行演变过程中额外发生随机突变。
Detailed antigenic analysis using a panel of monoclonal antibodies was carried out on the haemagglutinin antigen of 53 influenza B viruses isolated from an epidemic in a single school. Thirteen distinguishable antigenic groupings of influenza B viruses could be detected but 26 of the viruses were in two groups (III and IV) which co-existed during the entire epidemic. Antigenically distinguishable influenza B viruses were isolated from an epidemic in a second nearby school. Influenza B viruses isolated from the two schools could be further distinguished by different electrophoretic mobilities of NS1 polypeptides and of genes 1, 2, 3 and 6, whereas viruses from a single school epidemic were very closely related as regards these biochemical characteristics. The findings are consistent with the hypothesis that the outbreak was initiated by a single individual who excreted antigenic mutants of which predominantly two spread and coexisted during the epidemic, although the additional occurrence of random mutations during the evolution of the epidemic cannot be excluded.