Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM.
Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM.
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DOI：
10.1038/s41598-017-06988-1
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发表时间：
2017-07-28
影响因子：
4.6
通讯作者：
Fan Y
中科院分区：
文献类型：
--
作者：
Liu X;Tian J;Liu L;Zhu T;Yu X;Chu X;Yao B;Wu N;Fan Y
氟是普遍存在的，也是自然界中最活跃的非金属元素。虽然由于口腔卫生产品的广泛和长期应用，许多微生物已经产生了氟化物抗性，但这些微生物克服氟化物毒性的机制尚不完全清楚。在这项研究中，鉴定出一种耐氟菌株——阴沟肠杆菌FRM，它可以在氟化物浓度为4,000毫克/升的情况下良好生长。根据比较基因组学、氟胁迫下的转录组以及两个耐氟fosmid克隆的序列分析，发现基因组岛GI3对于耐氟性很重要。定量RT-PCR结果表明，GI3、ppaC、uspA、eno、gpmA、crcB和orf5249上的6个基因编码氟化物转运蛋白、氟化物抑制酶和通用应激蛋白，驻留在操纵子中，并通过氟化物核糖开关转录成由氟化物激活的两个mRNA。敲除和互补实验的结果表明，这些基因共同作用，为阴沟肠杆菌FRM提供了高氟化物抗性。这项研究阐明了这种高耐氟生物体的耐药机制，并扩大了我们对氟化物生物效应的理解。
Fluorine is ubiquitous and the most active non-metal element in nature. While many microorganisms have developed fluoride resistance as a result of the widespread and prolonged application of oral hygiene products, the mechanisms used by these organisms to overcome fluoride toxicity are incompletely understood. In this study, a fluoride-resistant strain, Enterobacter cloacae FRM, was identified which could grow well at a fluoride concentration of 4,000 mg/L. According to comparative genomics, transcriptome under fluoride stress, and sequence analyses of two fluoride-resistant fosmid clones, the genomic island GI3 was found to be important for fluoride resistance. The result of quantitative RT-PCR indicated that six genes on GI3, ppaC, uspA, eno, gpmA, crcB, and orf5249, which encode a fluoride transporter, fluoride-inhibited enzymes, and a universal stress protein, reside in an operon and are transcribed into two mRNAs activated by fluoride with a fluoride riboswitch. The results of knockout and complementation experiments indicated that these genes work together to provide high fluoride resistance to E. cloacae FRM. This study clarified the resistance mechanism of this high fluoride-resistant organism and has expanded our understanding of the biological effects of fluoride.
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影响因子：
4.4
作者：
Jiang, Yu;Chen, Biao;Yang, Sheng
通讯作者：
Yang, Sheng
影响因子：
3.7
作者：
Chen J;Tibroni N;Sauter D;Galaski J;Miura T;Alter G;Mueller B;Haller C;Walker BD;Kirchhoff F;Brumme ZL;Ueno T;Fackler OT
通讯作者：
Fackler OT
影响因子：
12.3
作者：
Weinberg Z;Wang JX;Bogue J;Yang J;Corbino K;Moy RH;Breaker RR
通讯作者：
Breaker RR
DOI：
10.1002/14651858.cd007868.pub2
发表时间：
2010-01-20
期刊：
The Cochrane database of systematic reviews
影响因子：
--
作者：
Walsh, Tanya;Worthington, Helen V;Shi, Xin
通讯作者：
Shi, Xin
影响因子：
14.9
作者：
Tatusov, RL;Natale, DA;Koonin, EV
通讯作者：
Koonin, EV