Yeast two-hybrid screening identified WDR77 as a novel interacting partner of TSC22D2
Yeast two-hybrid screening identified WDR77 as a novel interacting partner of TSC22D2
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酵母双杂交筛选确定 WDR77 是 TSC22D2 的新型相互作用伙伴
DOI：
10.1007/s13277-016-5113-z
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发表时间：
2016-09-01
期刊：
影响因子：
--
通讯作者：
Xiong, Wei
中科院分区：
文献类型：
--
作者：
Li, Qiao;Chen, Pan;Xiong, Wei
转化生长因子β刺激克隆22结构域家族成员2（TSC22D2）是TSC22D家族的一员，已被认为是一种肿瘤相关基因，但其功能仍不明确。为进一步探究其生物学作用，研究采用酵母双杂交筛选结合多种生物信息学工具，鉴定出44个TSC22D2蛋白的潜在相互作用蛋白，这些蛋白主要参与基因转录、细胞代谢和细胞周期调控。鉴于WD重复结构域蛋白77（WDR77）在细胞周期和肿瘤发生中的作用，以及其在酵母双杂交实验中的高检出频率，该蛋白被选作进一步验证对象。免疫沉淀和免疫荧光实验证实了TSC22D2与WDR77蛋白之间存在相互作用。我们的研究极大地拓展了TSC22D2的推测性蛋白相互作用网络，为深入了解TSC22D2和WDR77蛋白的生物学功能提供了新视角。
Transforming growth factor beta-stimulated clone 22 domain family, member 2 (TSC22D2), a member of the TSC22D family, has been implicated as a tumor-associated gene, but its function remains unknown. To further explore its biological role, yeast two-hybrid screening combined with multiple bioinformatics tools was used to identify 44 potential interacting partners of the TSC22D2 protein that were mainly involved in gene transcription, cellular metabolism, and cell cycle regulation. The protein WD repeat domain 77 (WDR77) was selected for further validation due to its function in the cell cycle and tumor development, as well as its high detection frequency in the yeast two-hybrid assay. Immunoprecipitation and immunofluorescence experiments confirmed an interaction between the TSC22D2 and WDR77 proteins. Our work greatly expands the putative protein interaction network of TSC22D2 and provides deeper insight into the biological functions of the TSC22D2 and WDR77 proteins.