High-throughput multi-antigen microfluidic fluorescence immunoassays
High-throughput multi-antigen microfluidic fluorescence immunoassays
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DOI：
10.2144/000112071
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发表时间：
2006-01-01
期刊：
影响因子：
2.7
通讯作者：
Anderson, WF
中科院分区：
文献类型：
--
作者：
Kartalov, EP;Zhong, JF;Anderson, WF
在这里，我们描述了高通量多抗原微流控荧光免疫分析系统的开发。 100 个室的聚二甲基硅氧烷 (PDMS) 芯片对 10 个样品中的每个样品执行最多 5 次测试。在这个特定的研究系统中，证明了检测的特异性，并制作了 C 反应蛋白 (CRP)、前列腺特异性抗原 (PSA)、铁蛋白和血管内皮生长因子 (VEGF) 的校准曲线。测量结果显示灵敏度等于或低于临床正常水平（抗原浓度低至 10 pM 时，信噪比 >8）。该芯片在所有测试中每个样品使用 100 nL。开发的系统是朝着科学研究中的衍生免疫测定应用和医学中的“即时护理”测试迈出的重要一步。
Here we describe the development of a high-throughput multi-antigen microfluidic fluorescence immunoassay system. A 100-chamber polydimethylsiloxane (PDMS) chip performs up to 5 tests for each of 10 samples. In this particular study system, the specificity of detection was demonstrated, and calibration curves were produced for C-reactive protein (CRP), prostate-specific antigen (PSA), ferritin, and vascular endothelial growth factor (VEGF). The measurements show sensitivity at and below clinically normal levels (with a signal-to-noise ratio >8 at as low as 10 pM antigen concentration). The chip uses 100 nL per sample for all tests. The developed system is an important step toward derivative immunoassay applications in scientific research and "point-of-care "testing in medicine.