Involvement of centrosomes in nuclear irregularity of thyroid carcinoma cells
Involvement of centrosomes in nuclear irregularity of thyroid carcinoma cells
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DOI：
10.1007/s00428-009-0802-2
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发表时间：
2009-08-01
期刊：
影响因子：
3.5
通讯作者：
Katoh, Ryohei
中科院分区：
文献类型：
--
作者：
Niu, Dongfeng;Murata, Shin-ichi;Katoh, Ryohei
核假性包涵体和核沟等核异常是甲状腺乳头状癌细胞的特征，被认为是组织病理学的重要诊断线索。我们观察了不同培养条件下甲状腺癌细胞系(KTC-1和TPC-1)的细胞核特征，并对细胞骨架分子进行了免疫细胞化学检查，以阐明甲状腺癌细胞核的形态发生。我们发现，在融合培养的细胞中出现了豆状核(BLN)和甜甜圈状核(DLNS)的核不规则，而在稀疏培养的细胞中则没有。在免疫细胞荧光分析中，甲状腺癌细胞的BLN凹陷处或DLN孔中经常可见代表中心体的成簇的γ-微管蛋白。综上所述，我们认为细胞间的接触可能会影响癌细胞系中的核变化，如BLN和DLN，中心体可能参与了这些核变化的形态发生过程。
Nuclear irregularities including nuclear pseudoinclusions and nuclear grooves are characteristic of papillary thyroid carcinoma cells and are regarded as important diagnostic clues in histopathology. We observed nuclear features of thyroid carcinoma cell lines (KTC-1 and TPC-1) in various culture conditions and performed immunocytochemical examinations for cytoskeleton molecules to clarify the morphogenesis of thyroid carcinoma nuclei. We found that nuclear irregularities presenting as bean-like nuclei (BLNs) and donut-like nuclei (DLNs) appeared in cells from confluent cultures, but not in cells from sparse cultures. On immunocytofluorescence analyses, clusters of gamma-tubulin, representing a centrosome, frequently localized at the indentation of BLNs or in the hole of DLNs of thyroid carcinoma cells. In conclusion, we suggest that cell-to-cell contact may affect nuclear changes such as BLNs and DLNs in cancer cell lines and that centrosomes may be involved in the morphogenetic process of these nuclear changes.