A novel leucine-rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection
A novel leucine-rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection
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一种新型富含亮氨酸的重复蛋白 CaLRR51 在辣椒对青枯菌感染的反应中充当正调节因子
DOI：
10.1111/mpp.12462
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发表时间：
2017-10-01
影响因子：
4.9
通讯作者：
He, Shuilin
中科院分区：
文献类型：
--
作者：
Cheng, Wei;Xiao, Zhuoli;He, Shuilin
富含亮氨酸重复序列(LRR)蛋白在植物防御反应中识别相应的配体和信号转导网络中起着重要作用。在此，我们鉴定并鉴定了一种新的辣椒LRR蛋白CaLRR51。它定位在细胞膜上，受青枯拉氏菌侵染(RSI)以及外源水杨酸(SA)、茉莉酸(JA)和乙烯利(ETH)的影响而转录上调。病毒诱导的CaLRR51基因沉默显著提高了辣椒对RSI的敏感性。相比之下，CaLRR51在辣椒植株中的瞬时过表达激活了类似过敏性反应(HR)的细胞死亡，并上调了与防御相关的标记基因PO2、HIR1、PR1、Def1和ACO1。此外，CaLRR51在转基因烟草中的异位过表达显著提高了烟草对RSI的抗性。在转基因烟草中，CaLRR51的过表达也增强了相应防御相关标记基因的转录表达，RSI增强了CaLRR51的转录表达。这些功能丧失和功能获得的分析表明，CaLRR51在辣椒对RSI的反应中起着积极的调节作用。此外，还发现CaLRR51的质膜靶向性需要信号肽和跨膜区，这是CaLRR51在植物免疫中发挥作用所必需的。
The leucine-rich repeat (LRR) proteins play important roles in the recognition of corresponding ligands and signal transduction networks in plant defence responses. Herein, a novel LRR protein from Capsicum annuum, CaLRR51, was identified and characterized. It was localized to the plasma membrane and transcriptionally up-regulated by Ralstonia solanacearum infection (RSI), as well as the exogenous application of salicylic acid (SA), jasmonic acid (JA) and ethephon (ETH). Virus-induced gene silencing of CaLRR51 significantly increased the susceptibility of pepper to RSI. By contrast, transient overexpression of CaLRR51 in pepper plants activated hypersensitive response (HR)-like cell death, and up-regulated the defence-related marker genes, including PO2, HIR1, PR1, DEF1 and ACO1. Moreover, ectopic overexpression of CaLRR51 in transgenic tobacco plants significantly enhanced the resistance to RSI. Transcriptional expression of the corresponding defence-related marker genes in transgenic tobacco plants was also found to be enhanced by the overexpression of CaLRR51, which was potentiated by RSI. These loss- and gain-of-function assays suggest that CaLRR51 acts as a positive regulator in the response of pepper to RSI. In addition, the putative signal peptide and transmembrane region were found to be required for plasma membrane targeting of CaLRR51, which is indispensable for the role of CaLRR51 in plant immunity.