Comparison of sequential drug release in vitro and in vivo.
Comparison of sequential drug release in vitro and in vivo.
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体外和体内连续药物释放的比较。
DOI：
10.1002/jbm.b.33472
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Puleo,DavidA
中科院分区：
文献类型：
--
作者：
Sundararaj,SharathC;Al-Sabbagh,Mohanad;Rabek,CherylL;Dziubla,ThomasD;Thomas,MarkV;Puleo,DavidA
药物递送装置的开发通常涉及表征体外释放性能，并固有地假设这将非常接近体内性能。然而，随着给药装置变得越来越复杂，例如具有顺序药物释放模式，确认体内特性与体外实现的预期“编程”相关是很重要的。在这项工作中，系统地比较了由邻苯二甲酸醋酸纤维素和Pluronic F‐127组成的多层结合聚合物体系中vitroandin的体内生物侵蚀和顺序释放。在磷酸盐缓冲盐水孵育期间对材料进行评估后，将装置植入大鼠的椎骨上。在植入后增加时间收获两种不同剂量和不同腐蚀速率的器械，并将其体内厚度损失、质量损失和药物释放情况与体外对照进行比较。在体外观察到的四种不同药物的顺序释放成功地转化为体内条件。结果表明，该装置的总腐蚀时间较长，四种药物的释放速度不同，药物在体内的释放速度先快后慢。许多生物可降解聚合物体外和体内药物释放的比较研究涉及单一药物，而本研究表明，植入后可以保持四种药物的顺序释放。©2015 Wiley期刊公司[J]中国生物医学工程学报，2016,31(4):559 - 561。
Development of drug‐delivery devices typically involves characterizingin vitrorelease performance with the inherent assumption that this will closely approximatein vivoperformance. Yet, as delivery devices become more complex, for instance with a sequential drug release pattern, it is important to confirm thatin vivoproperties correlate with the expected “programming” achievedin vitro. In this work, a systematic comparison betweenin vitroandin vivobiomaterial erosion and sequential release was performed for a multilayered association polymer system comprising cellulose acetate phthalate and Pluronic F‐127. After assessing the materials during incubation in phosphate‐buffered saline, devices were implanted supracalvarially in rats. Devices with two different doses and with different erosion rates were harvested at increasing times post‐implantation, and thein vivothickness loss, mass loss, and the drug release profiles were compared with theirin vitrocounterparts. The sequential release of four different drugs observedin vitrowas successfully translated toin vivoconditions. Results suggest, however, that the total erosion time of the devices was longer and that release rates of the four drugs were different, with drugs initially released more quickly and then more slowlyin vivo. Many comparative studies ofin vitroandin vivodrug release from biodegradable polymers involved a single drug, whereas this research demonstrated that sequential release of four drugs can be maintained following implantation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1302–1310, 2016.