DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways.
DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways.
复制标题
DOI：
10.1038/ncomms12433
复制
发表时间：
2016-08-12
影响因子：
16.6
通讯作者：
Wang E
中科院分区：
文献类型：
--
作者：
Jin Y;Liu H;Luo D;Yu N;Dong W;Wang C;Zhang X;Dai H;Yang J;Wang E
豆类与固氮菌或丛枝菌根真菌形成共生关系。这两个共生体的形成受一组共同的信号成分的调节，这些信号成分在宿主植物识别根瘤菌或菌根的下游起作用。这些途径的中心是钙和钙调蛋白依赖的蛋白激酶(CCaMK)-IPD3复合体，它在宿主细胞核内钙振荡后启动结节器官发生。然而，下行信令事件还没有被完全理解。在这里，我们表明紫花苜蓿蛋白质是赤霉酸信号的中央调节器，对根瘤菌的共生起积极的调节作用。在della突变体中，根瘤菌的定植受到了损害，我们提供的证据表明，della可以促进CCaMK-IPD3复合体的形成，并增加IPD3的磷酸化状态。DELLAS还可以与NSP2-NSP1相互作用，增强结节因子诱导基因在原生质体中的表达。我们发现della能够连接包含IPD3和NSP2的蛋白质复合体。我们的结果提示了一个调控根瘤共生的转录框架。豆类和固氮菌之间的共生关系受CCaMK-IPD3蛋白质复合体的调节，CCaMK-IPD3蛋白质复合体促进根瘤起始。在这里，金等人。认为Della蛋白通过促进IPD3的磷酸化和作为转录的正调控因子对结瘤起积极的调节作用。
Legumes form symbiotic associations with either nitrogen-fixing bacteria or arbuscular mycorrhizal fungi. Formation of these two symbioses is regulated by a common set of signalling components that act downstream of recognition of rhizobia or mycorrhizae by host plants. Central to these pathways is the calcium and calmodulin-dependent protein kinase (CCaMK)–IPD3 complex which initiates nodule organogenesis following calcium oscillations in the host nucleus. However, downstream signalling events are not fully understood. Here we show that Medicago truncatula DELLA proteins, which are the central regulators of gibberellic acid signalling, positively regulate rhizobial symbiosis. Rhizobia colonization is impaired in della mutants and we provide evidence that DELLAs can promote CCaMK–IPD3 complex formation and increase the phosphorylation state of IPD3. DELLAs can also interact with NSP2–NSP1 and enhance the expression of Nod-factor-inducible genes in protoplasts. We show that DELLA is able to bridge a protein complex containing IPD3 and NSP2. Our results suggest a transcriptional framework for regulation of root nodule symbiosis. Symbiotic associations between legumes and nitrogen-fixing bacteria are regulated by a CCaMK-IPD3 protein complex which promotes nodule initiation. Here, Jin et al. propose that DELLA proteins positively regulate nodulation by enhancing phosphorylation of IPD3 and acting as a positive regulator of transcription.