The structure of the yeast plasma membrane SNARE complex reveals destabilizing water-filled cavities
The structure of the yeast plasma membrane SNARE complex reveals destabilizing water-filled cavities
复制标题
DOI：
10.1074/jbc.m707912200
复制
发表时间：
2008-01-11
影响因子：
4.8
通讯作者：
Brunger, Axel T.
中科院分区：
文献类型：
--
作者：
Strop, Pavel;Kaiser, Stephen E.;Brunger, Axel T.
SNARE蛋白形成复合物，导致所有真核细胞中囊泡、细胞器和质膜之间的膜融合。我们报告的1.7埃分辨率结构的陷阱复合物介导的胞吐作用在质膜的酵母酿酒酵母。与其神经元和内体同源物相似，S。酿酒酵母SNARE复合物形成平行的四螺旋束，其中心是离子层。色葡萄酿酒酵母SNARE复合物在离子层附近表现出增加的螺旋弯曲，在复合物核心中含有充满水的空腔，并且相对于哺乳动物SNARE复合物表现出降低的热稳定性。诱变实验表明，充水空腔导致S.酿酒酵母复合体
SNARE proteins form a complex that leads to membrane fusion between vesicles, organelles, and plasma membrane in all eukaryotic cells. We report the 1.7 angstrom resolution structure of the SNARE complex that mediates exocytosis at the plasma membrane in the yeast Saccharomyces cerevisiae. Similar to its neuronal and endosomal homologues, the S. cerevisiae SNARE complex forms a parallel four-helix bundle in the center of which is an ionic layer. The S. cerevisiae SNARE complex exhibits increased helix bending near the ionic layer, contains water-filled cavities in the complex core, and exhibits reduced thermal stability relative to mammalian SNARE complexes. Mutagenesis experiments suggest that the water-filled cavities contribute to the lower stability of the S. cerevisiae complex.