Intrahepatic interleukin-8 production during disease progression of chronic hepatitis C
Intrahepatic interleukin-8 production during disease progression of chronic hepatitis C
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DOI：
10.1016/j.canlet.2006.10.028
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发表时间：
2007-06-18
期刊：
影响因子：
9.7
通讯作者：
Kaneko, Shuichi
中科院分区：
文献类型：
--
作者：
Tachibana, Yoshiya;Nakamoto, Yasunari;Kaneko, Shuichi
本研究旨在通过测量IL-8、单核细胞趋化蛋白-1 （MCP-1）和巨噬细胞炎症蛋白-1 α (MIP-1 α)的产生，探讨趋化因子在慢性丙型肝炎和肝细胞癌（HCC）发病中的作用。建立了固相三明治酶联免疫吸附法（ELISA）来定量血清中趋化因子的浓度。使用特异性单克隆抗体免疫组织化学方法评估肝组织中趋化因子的表达。随着慢性肝炎病情的加重，血清IL-8水平逐渐升高。此外，在肝细胞癌（HCC）患者中，IL-8浓度与HCC宏观分期呈正相关，与生存期长短呈负相关。结果表明，IL-8的产生可能会增加慢性丙型肝炎肝细胞的恶性转化(c)。版权所有。
The current study was designed to investigate the contribution of chemokines to the pathogenesis of chronic hepatitis C and hepatocellular carcinoma (HCC) by measuring the production of IL-8, monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 alpha (MIP-1 alpha). A solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) was established to quantitate serum concentrations of the chemokines. Expression of chemokines in liver tissues was evaluated immumohistochemically using specific monoclonal antibodies. As the severity of chronic hepatitis escalated, serum IL-8 levels increased progressively. Moreover, in the hepatocellular carcinoma (HCC) patients, IL-8 concentrations were positively correlated with the macroscopic staging of HCC, and inversely correlated with the duration of the survival periods. The results demonstrate that IL-8 production may be augmented upon the malignant transformation of hepatocytes in chronic hepatitis C. (c) 2007 Elsevier Ireland Ltd. All rights reserved.