Raman spectroscopy detects deterioration in biomechanical properties of bone in a glucocorticoid-treated mouse model of rheumatoid arthritis
Raman spectroscopy detects deterioration in biomechanical properties of bone in a glucocorticoid-treated mouse model of rheumatoid arthritis
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DOI：
10.1117/1.3613933
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发表时间：
2011-08-01
影响因子：
3.5
通讯作者：
Berger, Andrew J.
中科院分区：
文献类型：
--
作者：
Maher, Jason R.;Takahata, Masahiko;Berger, Andrew J.
虽然糖皮质激素经常用于炎症性疾病（如类风湿性关节炎）的对症治疗，但长期糖皮质激素暴露是医生诱导的骨质疏松症的主要原因，并使患者处于骨折的高风险中。为了研究糖皮质激素暴露的生物化学效应以及它们如何影响骨的生物力学特性，从糖皮质激素和安慰剂处理的野生型小鼠和类风湿性关节炎转基因小鼠模型的离体tibridium获得拉曼光谱。由于给药方案和小鼠基因型，观察到统计学显著性光谱差异。这些差异归因于整体骨矿物质组成的变化，以及胫骨皮质骨中磷酸盐矿化的程度。此外，使用偏最小二乘回归生成每个胫骨生物力学强度的基于拉曼的预测，如通过扭转测试量化的。基于拉曼光谱的预测与微计算机断层扫描衍生参数产生的预测一样准确，并且比临床使用的骨矿物质密度参数更准确。这些结果表明，拉曼光谱可能是一个有价值的工具，监测骨生物化学的研究，如骨质疏松症，包括测试药物正在开发，以打击这些疾病。(C)2011年，美国光电仪器工程师学会（SPIE）。[DOI：10.1117/1.3613933]
Although glucocorticoids are frequently prescribed for the symptomatic management of inflammatory disorders such as rheumatoid arthritis, extended glucocorticoid exposure is the leading cause of physician-induced osteoporosis and leaves patients at a high risk of fracture. To study the biochemical effects of glucocorticoid exposure and how they might affect biomechanical properties of the bone, Raman spectra were acquired from ex vivo tibiae of glucocorticoid- and placebo-treated wild-type mice and a transgenic mouse model of rheumatoid arthritis. Statistically significant spectral differences were observed due to both treatment regimen and mouse genotype. These differences are attributed to changes in the overall bone mineral composition, as well as the degree of phosphate mineralization in tibial cortical bone. In addition, partial least squares regression was used to generate a Raman-based prediction of each tibia's biomechanical strength as quantified by a torsion test. The Raman-based predictions were as accurate as those produced by inicrocomputed tomography derived parameters, and more accurate than the clinically-used parameter of bone mineral density. These results suggest that Raman spectroscopy could be a valuable tool for monitoring bone biochemistry in studies of bone diseases such as osteoporosis, including tests of drugs being developed to combat these diseases. (C) 2011 society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3613933]