Cloning, characterization and functional analysis of dctn5 in immune response of Chinese tongue sole (Cynoglossus semilaevis)
Cloning, characterization and functional analysis of dctn5 in immune response of Chinese tongue sole (Cynoglossus semilaevis)
复制标题
半滑舌鳎 (Cynoglossus semilaevis) 免疫应答中 dctn5 的克隆、表征及功能分析
DOI：
10.1016/j.fsi.2018.04.007
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发表时间：
2018-06
影响因子：
4.7
通讯作者：
Chen Songlin
中科院分区：
文献类型：
--
作者：
Wei Min;Xu Wenteng;Li Kunming;Chen Yadong;Wang Lei;Meng Liang;Zhao Fazhen;Chen Songlin
在哺乳动物中，微管依赖性运输可以参与免疫反应，其中运动蛋白被认为在此过程中发挥重要作用，而在鱼类中的相关研究很少。本研究通过前期免疫QTL筛选获得了用于对接运动蛋白的dyactin复合物亚基dctn5。克隆并鉴定了两个 dctn5 转录变体的全长 cDNA（分别命名为 dctn5_tv1 和 dctn5_tv2）。组织分布显示，dctn5_tv1在免疫组织（皮肤）中分布广泛，且转录水平较高，而dctn5_tv2主要在性腺中检测到，在其他组织中检测水平很低。时程表达分析表明，哈维弧菌攻击后，dctn5_tv1 在鳃、肠、皮肤、脾和肾中表达上调。此外，重组Dctn5tv1由于与细菌细胞结合，对大肠杆菌和无乳链球菌表现出高抗菌活性。综上所述，这些数据表明Dctn5_tv1参与中华舌鳎细菌入侵的免疫反应。
In mammals, microtubule-dependent trafficking could participate the immune response, where the motor proteins are suggested to play an important role in this process, while the related study in fish was rare. In this study, dctn5, a subunit of dyactin complex for docking motor protein, was obtained by previous immune QTL screening. The full-length cDNAs of two dctn5 transcript variants were cloned and identified (named dctn5_tv1 and dctn5_tv2, respectively). Tissue distribution showed that dctn5_tv1 was widely distributed and high transcription was observed in immune tissue (skin), while dctn5_tv2 was predominantly detected in gonad and very low in other tissues. Time-course expression analysis revealed that dctn5_tv1 could be up-regulated in gill, intestine, skin, spleen, and kidney after Vibrio harveyi challenge. Moreover, recombinant Dctn5tv1 exhibited high antimicrobial activity against Escherichia coli and Streptococcus agalactiae due to binding to bacteria cells. Taken together, these data suggest Dctn5_tv1 is involved in immune response of bacterial invasion in Chinese tongue sole.
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