Inhibitory effects of intact silkworm sericin on bacterial proliferation
Inhibitory effects of intact silkworm sericin on bacterial proliferation
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完整蚕丝胶对细菌增殖的抑制作用
DOI：
10.1080/00405000.2020.1787302
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Kotani Eiji
中科院分区：
文献类型：
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作者：
Matsumoto Erica;Takaki Keiko;Maruta Rina;Mori Hajime;Kotani Eiji
丝胶蛋白来源于普通蚕（家蚕）的丝线蛋白的外层，并且充当胶以固定发育中的茧的纤维。尽管丝胶蛋白在包括组织工程和化妆品开发在内的各个领域都有潜在的用途，但完整的丝胶蛋白的生物学应用还有待确定。我们以前开发的转基因蚕生产的茧完全由丝胶蛋白，我们可以很容易地从它制备完整的丝胶蛋白的溶液。在这里，我们报告了完整的丝胶对细菌增殖的影响。完整的丝胶蛋白不能杀死细菌，但大肠杆菌培养基中的菌落大小在完整的丝胶蛋白存在下培养时可减少高达90%。此外，完整的丝胶蛋白的处理减少了肠道沙门氏菌的菌落大小，表明完整的丝胶蛋白对革兰氏阴性菌的生长具有潜在的抑制作用。然而，革兰氏阳性菌，包括金黄色葡萄球菌和枯草芽孢杆菌，不受完整的丝胶。因此，我们的研究结果表明，不同细菌的细胞壁结构可能会影响其敏感性的抑制作用的丝胶。
Sericins are derived from the outer layer of silk thread proteins of the common silkworm (Bombyx mori) and act as a glue to fix fibers of the developing cocoon. Although sericin has potential uses in various fields, including tissue engineering and the development of cosmetics, biological applications of intact sericin have yet to be identified. We previously developed transgenic silkworms that produce cocoons solely composed of sericin, from which we can readily prepare a solution of intact sericin. Here we report the effects of intact sericin on the proliferation of bacteria. The intact sericin does not kill bacteria, but colony size ofEscherichia colion culture media was reduced by up to 90% when cultured in the presence of intact sericin. In addition, treatment of intact sericin reduced colony size ofSalmonella enterica, indicating a potential inhibitory effect of intact sericin on growth in Gram-negative bacteria. However, Gram-positive bacteria, includingStaphylococcus aureusandBacillus subtilis, were not affected by intact sericin. Thus, our results indicate that the cell wall structure of different bacteria may influence their sensitivity to the inhibitory effects of sericin.