SELECTIVE PROLIFERATION OF NORMAL HUMAN MELANOCYTES INVITRO IN THE PRESENCE OF PHORBOL ESTER AND CHOLERA-TOXIN
SELECTIVE PROLIFERATION OF NORMAL HUMAN MELANOCYTES INVITRO IN THE PRESENCE OF PHORBOL ESTER AND CHOLERA-TOXIN
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DOI：
10.1073/pnas.79.6.2018
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
MARKO, O
中科院分区：
文献类型：
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作者：
EISINGER, M;MARKO, O
通过在培养基中使用佛波醇12-肉豆蔻酸酯13-乙酸酯（10 ng/ml），从表皮单细胞悬浮液获得几乎完全由人黑素细胞组成的培养物。在此浓度下，佛波酯对人角质形成细胞有毒，但对黑素细胞无毒。当接种密度为最佳时（0.8-2 × 10 - 6），104/cm ~ 2）和同时含有佛波醇酯和霍乱毒素的培养基中，黑素细胞广泛增殖。在此条件下，人黑素细胞可以在体外连续传代并大量生长。因此，这种细胞培养系统可用于回答与色素细胞生物学相关的基本问题，并可作为恶性黑素细胞研究的对照。
Cultures consisting almost entirely of human melanocytes were obtained from epidermal single-cell suspensions by using phorbol 12-myristate 13-acetate (10 ng/ml) in the culture medium. At this concentration, phorbol ester is toxic to human keratinocytes but not to melanocytes. When the seeding density was optimal (0.8-2 .times. 104/cm2) and the medium contained both phorbol ester and cholera toxin, melanocytes proliferated extensively. Under these conditions, human melanocytes could be passaged serially in vitro and grown in quantity. This cell culture system can thus be used to answer basic questions related to pigment cell biology and may serve as a control for studies of malignant melanocytes.