EVIDENCE THAT CHOLINERGIC AXONS FROM THE PARABRACHIAL REGION OF THE BRAIN-STEM ARE THE EXCLUSIVE SOURCE OF NITRIC-OXIDE IN THE LATERAL GENICULATE-NUCLEUS OF THE CAT
EVIDENCE THAT CHOLINERGIC AXONS FROM THE PARABRACHIAL REGION OF THE BRAIN-STEM ARE THE EXCLUSIVE SOURCE OF NITRIC-OXIDE IN THE LATERAL GENICULATE-NUCLEUS OF THE CAT
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DOI：
10.1002/cne.903340307
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发表时间：
1993-08-15
影响因子：
2.5
通讯作者：
SHERMAN, SM
中科院分区：
文献类型：
--
作者：
BICKFORD, ME;GUNLUK, AE;SHERMAN, SM
我们调查的来源，轴突和终端在猫的外侧膝状体核染色阳性NADPH-黄递酶。这种染色的功能意义在于NADPH-黄递酶与一氧化氮合成酶相同，因此认为它可以揭示使用一氧化氮作为神经调节剂的细胞和轴突。在外侧膝状体和邻近的周膝状体核内，轴突和终末的致密网络被标记为NADPH-黄递酶。NADPH黄递酶染色的模式与胆碱乙酰转移酶（ChAT）染色的模式非常相似，这表明这些轴突和终末的来源可能是脑干的臂旁区，因为这为外侧膝状体核提供了主要的胆碱能输入。在臂旁轴突投射到的大脑其他区域，NADPH-黄递酶和ChAT也有类似的染色模式。此外，在臂旁区域内的NADPH-黄递酶和ChAT的细胞染色模式几乎是相同的。然而，ChAT和NADPH-黄递酶染色的臂旁区之间的关系不是胆碱能神经元的一般属性。其他胆碱能细胞和轴突，如滑车神经，眼神经和核，和parabigeminal核，都标记密集的ChAT，染色差或根本没有NADPH-黄递酶。重要的是，向外侧膝状体核提供胆碱能输入的二叠旁核没有标记NADPH黄递酶的细胞。我们用双标记法进一步鉴定了外膝状体中NADPH-黄递酶染色的来源。我们发现臂旁细胞共同定位NADPH-黄递酶和ChAT。脑干中的去甲肾上腺素能细胞和肾上腺素能细胞也支配外侧膝状体核，但我们发现，没有这些共同定位的NADPH-黄递酶。最后，通过结合NADPH-黄递酶组织化学与逆行标记的细胞，项目的外侧膝状体核，我们发现，臂旁区的胆碱能细胞基本上是唯一的来源，NADPH-黄递酶在外侧膝状体核。因此，我们得出结论，从臂旁区的细胞，支配外侧膝状体核使用乙酰胆碱和一氧化氮的神经传递，这几乎是唯一的传入输入到这个地区，使用一氧化氮。(C)1993 Wiley-Liss，Inc.
We investigated the source of axons and terminals in the cat's lateral geniculate nucleus that stain positively for NADPH-diaphorase. The functional significance of such staining is that NADPH-diaphorase is identical to the enzyme nitric oxide synthetase, and thus it is thought to reveal cells and axons that use nitric oxide as a neuromodulator. Within the lateral geniculate and adjacent perigeniculate nuclei, a dense network of axons and terminals is labeled for NADPH-diaphorase. The pattern of NADPH-diaphorase staining here is remarkably similar to that of choline acetyltransferase (ChAT) staining, suggesting that the source of these axons and terminals might be the parabrachial region of the brainstem because this provides the major cholinergic input to the lateral geniculate nucleus. In other areas of the brain to which parabrachial axons project, there is also a similar staining pattern for NADPH-diaphorase and ChAT. Furthermore, the patterns of cell staining within the parabrachial region for NADPH-diaphorase and ChAT are virtually identical. However, the relationship between ChAT and NADPH-diaphorase staining for the parabrachial region is not a general property of cholinergic neurons. Other cholinergic cells and axons, such as the trochlear nerve, the oculomotor nerve and nucleus, and the parabigeminal nucleus, which all label densely for ChAT, stain poorly or not at all for NADPH-diaphorase. It is significant that the parabigeminal nucleus, which provides a cholinergic input to the lateral geniculate nucleus, has no cells that label for NADPH-diaphorase. We used double labeling methods to identify further the source of NADPH-diaphorase staining in the lateral geniculate nucleus. We found that parabrachial cells co-localize NADPH-diaphorase and ChAT. Noradrenergic and serotoninergic cells in the brainstem also innervate the lateral geniculate nucleus, but we found that none of these co-localize NADPH-diaphorase. Finally, by combining NADPH-diaphorase histochemistry with retrograde labeling of cells that project to the lateral geniculate nucleus, we found that the cholinergic cells of the parabrachial region are essentially the sole source of NADPH-diaphorase in the lateral geniculate nucleus. We thus conclude that cells from the parabrachial region that innervate the lateral geniculate nucleus use both acetylcholine and nitric oxide for neurotransmission, and that this is virtually the only afferent input to this region that uses nitric oxide. (C) 1993 Wiley-Liss, Inc.