Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg.
Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg.
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DOI：
10.1073/pnas.86.18.7038
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发表时间：
1989-09
影响因子：
11.1
通讯作者：
S. O'Keefe;H. Wolfes;A. Kiessling;G. Cooper
中科院分区：
文献类型：
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作者：
S. O'Keefe;H. Wolfes;A. Kiessling;G. Cooper
采用注射反义寡核苷酸的方法研究c-mos在小鼠卵母细胞中的功能。注射反义c-mos寡核苷酸的卵母细胞完成了第一次减数分裂，但未能启动减数分裂II。相反，c-mos功能的丧失导致染色体解凝聚，减数分裂I后的细胞核重组，分裂成两个细胞。因此，c-mos是小鼠卵母细胞成熟过程中减数分裂II所必需的。
Injection of antisense oligonucleotides was used to investigate the function of c-mos in murine oocytes. Oocytes injected with antisense c-mos oligonucleotides completed the first meiotic division but failed to initiate meiosis II. Instead, loss of c-mos function led to chromosome decondensation, reformation of a nucleus after meiosis I, and cleavage to two cells. Therefore, c-mos is required for meiosis II during murine oocyte maturation.