Transcriptional profiling of MEF2-regulated genes in human neural progenitor cells derived from embryonic stem cells.
Transcriptional profiling of MEF2-regulated genes in human neural progenitor cells derived from embryonic stem cells.
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来自胚胎干细胞的人类神经祖细胞中MEF2调节基因的转录分析。
DOI：
10.1016/j.gdata.2014.10.022
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发表时间：
2015-03-01
期刊：
影响因子：
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通讯作者：
Lipton, Stuart A
中科院分区：
文献类型：
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作者：
Chan, Shing Fai;Huang, Xiayu;McKercher, Scott R;Zaidi, Rameez;Okamoto, Shu-Ichi;Nakanishi, Nobuki;Lipton, Stuart A
肌细胞增强因子2(MEF2)家族转录因子在脑中高度表达，是神经元存活、分化和突触可塑性的关键决定因素。然而，MEF2调控基因的全基因组转录图谱尚未完全阐明，特别是在神经干细胞阶段。在这里，我们报告了微阵列分析的结果，比较了从表达控制载体的胚胎干细胞中分离的人类神经前体/干细胞(HNPC)和表达成分活性形式的MEF2(MEF2CA)的前体细胞的mRNAs，该形式增加了MEF2的活性。使用Illumina Human HT-12和V4.0 Expression BeadChip(GEO#：GSE57184)进行微阵列实验。通过比较载体对照细胞和MEF2CA细胞，微阵列分析确定了1880个差异表达的独特基因。其中，1121个基因表达上调，759个基因表达下调。我们的结果为确定hNPC中MEF2的转录靶点提供了有价值的资源。
The myocyte enhancer factor 2 (MEF2) family of transcription factors is highly expressed in the brain and constitutes a key determinant of neuronal survival, differentiation, and synaptic plasticity. However, genome-wide transcriptional profiling of MEF2-regulated genes has not yet been fully elucidated, particularly at the neural stem cell stage. Here we report the results of microarray analysis comparing mRNAs isolated from human neural progenitor/stem cells (hNPCs) derived from embryonic stem cells expressing a control vector versus progenitors expressing a constitutively-active form of MEF2 (MEF2CA), which increases MEF2 activity. Microarray experiments were performed using the Illumina Human HT-12 V4.0 expression beadchip (GEO#: GSE57184). By comparing vector-control cells to MEF2CA cells, microarray analysis identified 1880 unique genes that were differentially expressed. Among these genes, 1121 genes were up-regulated and 759 genes were down-regulated. Our results provide a valuable resource for identifying transcriptional targets of MEF2 in hNPCs.